大鼠肝癌细胞株细胞的同步化及其检测

Synchronization and Detection of Rat Hepatocellular Carcinoma Cell Line Cells

细胞增殖分裂是细胞最基本的生理活动。影响细胞增殖分裂归根到底是影响细胞周期的运行 ,细胞周期失控的超常快速运行将导致细胞癌变 ;停滞不前则常是细胞衰老、凋亡的前奏。以大鼠肝癌细胞株 (HTC)的细胞为研究对象 ,探讨了HTC细胞的同步化方法 ,并经流式细胞仪测定同步率 ,结果表明 :在HTC细胞对数生长期 ,以胸腺嘧啶核苷和秋水仙碱顺序阻断法及胸腺嘧啶核苷双阻断法可分别获得同步率为 72 .10 %的G1期和 98.94%的S期HTC细胞。经多次实验证明 ,这一结果稳定。

The proliferation and division of cells are the most basic reaction. The effects of this reaction are the fact that the cell cycle was been influenced. Normal cells will change into tumor cells when its cell cycle running out of control; on the other hand, it will become feeble and die when its cell cycle stopping. Methods of synchronization and detection with flow cytometer of rat hepatocellular carcinoma cell line (HTC) are established in our laboratory. The results show that: as HTC cell is in the logarithmic period, the synchronous 72.10% G 1 phase and 98.94% S phase cells are achieved through thymine 2 desoryriboside and cochicine sequential blockage method and double thymine 2 desoryriboside blockage method respectively. It is proved that those results ware stable and reliable.