大豆甾醇的微生物降解及酶抑制剂的选择

Microbial Transformation of Soybean Sterol and the Selection of Enzyme Inhibitors

测定了诺卡氏菌（Ｎｏｃａｒｄｉａ）、分枝杆菌（Ｍｙｃｏｂ ａｃｔｅｒｉｕｍ）和节杆菌（Ａｒｔｈｒｏｂ ａｃｔｅｒ）对大豆甾醇的降解能力及Ｎｉ２＋，Ｃｏ２＋，Ｍｎ２＋，α、α’－联吡啶、邻菲罗琳等酶抑制剂对△１，４－雄甾二烯－３， １７－二酮（ＡＤＤ）积累的影响．实验结果表明：节杆菌 Ａ－６在６０× １０－６硫酸镍存在下，使大豆甾醇的转化率达７０％以上，ＡＤＤ产率达３０％左右。

The ability of microorganisms (Nocardia, Mycobacterium and Arthrobacter) is studied to degrade the soybean sterol, the influence of enzyme inhibitors, which inclus Ni2+. Co2+, Mn2+, a. a' - dipyridine, l, 10 - phenanthroline and 8 -hydroxyl - quinoline, on the accumulation of △1, 4- androst - diene - 3, 17 - dione (ADD). The results showed that the transformation rate of soybean sterol was 70% and the yield of ADD (in g/g) was 30% when Anthrobacter A- 6 was used and in the presence of 60 x 10-6 NiSO4, as an enzyme inhibitor.