桂皮醛对口腔鳞状细胞癌细胞抑制作用的体外研究

Effects of cinnamaldehyde on oral squamous carcinoma cell in vitro

目的体外研究中药桂皮醛对口腔鳞状细胞癌细胞增殖的抑制作用。方法以3种人口腔鳞状细胞癌细胞株为研究对象,采用噻唑蓝(MTT)比色法观察桂皮醛不同质量浓度、不同作用时间下对各细胞株增殖的影响。应用SPSS 16.0软件对数据进行方差分析。结果与空白对照组相比,256、128、64、32 mg.L-1质量浓度组的桂皮醛对人口腔鳞状细胞癌细胞的增殖表现出明显抑制作用,且抑制作用随着药物质量浓度的升高而加强,呈现明显的剂量-效应关系。药物作用时间24 h时,作用质量浓度16 mg.L-1时,对人舌鳞状细胞癌Tca8113细胞株抑瘤率达到58.33%;作用质量浓度为32 mg.L-1时对KB细胞及人颊黏膜鳞状细胞癌BCaCD885细胞株的抑制率分别为66.08%、76.61%,其抑制率随着药物作用时间的延长而增加。结论桂皮醛对口腔鳞状细胞癌细胞增殖的抑制作用与质量浓度和作用时间有关,中、高浓度的桂皮醛对3种人口腔鳞状细胞癌细胞增殖均具有明显的抑制作用,在一定程度上肯定了桂皮醛的抗肿瘤作用。

Objective To investigate the inhibiting in vitro effect of cinnamaldehyde on human oral squamous carcinoma cell. Methods Using methyl thiazolyl tetrazolium （MTY） to assay the effect of cinnamaldehyde on hu- man tongue carcinoma cell lines（Tca8113）, human buccal squamous carcinoma cell lines（BCaCD885）, human oral epithelial carcinoma KB cell in different concentrations. The final concentrations of cinnamaldehyde were from 8 to 256 mg.L-1, respectively. The cells were cultured in different concentrations, and the cinnamaldehyde continuously contacted with the cells for 24, 48 and 72 h, respectively, we compared the efficacy of cinnamaldehyde in dif- ferent concentration groups during different time quantum. Differences between data obtained were statistically analyzed using analysis of variance （ANOVA） in SPSS 16.0. Results When the concentration of cinnamicaldehyde were from 32 to 256 mg.L-1, it had a significant inhibition on the proliferation of oral cancer cells in vitro, and with a increasing concentration of cinnamicaldehyde, the efficacy of inhibition became more significant, which showed an obvious dosage-efficacy relationship. When cinnamaldehyde acted 24 hours or its concentration was 16mg.L-1, the inhibition rate was 58.33% for the human tongue squamous cell carcinoma cell line Tca8113; when the concentration was on the level of 32 mg.L-1, the inhibition rate were 66.08%, 76.61% respectively for human oral epithelial carcinoma KB cell and human buccal squamous carcinoma cell BCaCD885, respectively. Conclusion In the medium-high （from 32 to 256 mg.L-1） concentrations of cinnamaldehyde showed remarkable cytostatic activity in terms of inhibition the proliferation of oral squamous carcinoma epithelial cell.