Cyclin D1和P16在增生性瘢痕成纤维细胞中的表达及意义

Expression and significance of Cyclin D1 and P16 in fibroblasts of hypertrophic scars

目的观察增生性瘢痕中成纤维细胞中Cyclin D1、P16的表达,探讨其与正常皮肤组织之间的差异关联性。方法12例增生性瘢痕为实验组,正常皮肤组织作为对照组,进行成纤维细胞培养,通过SABC免疫组化检测两组中CyclinD1、P16的表达。结果 Cyclin D1在对照组成纤维细胞表达为阴性,实验组的阳性表达率为83.3%,两者比较差异具有统计学意义(P<0.01);与对照组P16阴性比较,增生性瘢痕成纤维细胞的P16阳性表达率为75.0%,差异具有统计学意义(P<0.01)。结论 Cyclin D1和P16作为一对细胞周期的正负调控因子的表达失衡是增生性瘢痕的发展的重要机制。

Objective To observe the Cyclin D1, P16 expression in hypertrophic scar fibroblasts, and to explore the differ- ences between normal skin tissue correlation. Methods All of 12 cases of hypertrophic scars for the experimental group, normal skin tissue as a control group, the two groups of Cyclin D1 and P16 expression were detected by SABC immunohis- tochemical. Results Cyclin D1 of fibroblasts in the control expression of negative, positive expression rate was 83.3% of the experimental group, there was a statistically significant difference（P 〈 0.01 ）;compared with the control group PI6 negative P16 positive expression of hypertrophic scar fibroblasts rate of 75.0%, there was a significant difference （P 〈 0.01 ）. Con- clusion Cyclin D1 and P16 cell cycle as a pair of positive and negative regulatory factor expression imbalance is an im- portant mechanism of the development of hypertrophic scars.