不同生长时期人参根中3种氧化还原同工酶分析

Analysis on three kinds of oxidoreductase isozymes in ginseng root at different growth periods

目的研究不同生长时期人参根中3种氧化还原同工酶的变化规律。方法以5年生人参根为供试材料,采用聚丙烯酰胺凝胶电泳(PAGE)法得到人参根超氧化物歧化酶(SOD)同工酶、过氧化氢酶(CAT)同工酶和细胞色素氧化酶(CYT)同工酶的电泳胶片,并生成指纹图谱叠加图,对不同生长时期人参根中上述3种氧化还原同工酶图谱进行分析。结果不同生长时期人参根中SOD同工酶和CYT同工酶的酶带条数存在明显差别,而CAT同工酶的酶带条数无明显变化,3种同工酶均具有各自的主要特征酶带。结论 SOD同工酶和CYT同工酶在人参果后参根生长期和枯萎期活性升高,CAT同工酶在人参生长发育的各个时期活性相对稳定。

Objective To study the variation rules of the three kinds of oxidoreductase isozymes in ginseng root at different growth periods.Methods Taking five-year old ginseng roots as materials,the electrophoresis films of superoxide dismutase（SOD） isozyme,catalase（CAT） isozyme,and cytochrome oxidase（CYT） isozyme were obtained by using the polyacrylamide gel electrophoresis（PAGE） method.The overlapped fingerprint was generated and the three redox isoenzymes of ginseng roots at different growth periods were analyzed.Results The enzyme band number of SOD isozyme and CYT isozyme in ginseng root at different growth periods showed obvious differences,but the enzyme bands numberof CAT isozyme demonstrated no significant change.All the three isozymes had main characteristic bands of its own.Conclusion Activities of SOD isozyme and CYT isozyme are increased in ginseng root growing after fruit stage and withering stage,while the activity of CAT isozyme is relatively stable in each period of ginseng during the growth and development.