灰飞虱体内Wolbachia的检测及三种稻飞虱Wolbachia的wsp基因差异分析

Detection of Wolbachia in Laodelphax striatellus and analysis of Wolbachia wsp gene between three species of the rice planthopper

采用PCR方法对灰飞虱感染Wolbachia情况进行检测,并对Wolbachia的wsp基因进行序列测定和系统发育分析,以明确各地区Wolbachia的差异及三种稻飞虱体内Wolbachia的差异。结果表明,12省份21地区灰飞虱群体Wolbachia平均感染率达到96.41%。各地灰飞虱体内Wolbachia不存在明显的地理种群分化。与褐飞虱及白背飞虱体内Wolbachia的wsp序列对比,灰飞虱与白背飞虱的序列一致,而与褐飞虱存在较大差异(相似性83.39%),处于不同的系统进化簇。表明感染白背飞虱与灰飞虱的Wolbachia处于相同株系,而感染褐飞虱的Wolbachia处于另一株系。

To identify the geographical genetic differentiation of Wolbachia in the small brown planthopper （Lao- delphax striatellus, SBPH） and it＇s variation among three species of the rice planthopper, we detected the Wolbahcia outer surface protein （wsp） gene in SBPH by the PCR method, and the sequences were analyzed by phylogenetic analysis. The results showed that the average infection rate of Wolbachia in SBPH collected from 21 regions in 12 provinces was 96.41%. There was no geographical genetic differentiation in Walachia wsp gene. Wolbachia in the SBPH and the white-backed planthopper （Sogatella furcifera, WBPH） shared the same homology in wsp gene sequences, but differences were found in the brown planthopper （Nilaparvata lugens, BPH）, with a similarity of 83.39%. The results gained in this study indicated that SBPH and WBPH might be infected by the same strain of Wolbachia, while BPH by a different strain.