p63与大鼠全脑缺血后神经元凋亡的关系

Relationship between p63 and neuronal apoptosis after global cerebral ischemia in rats

目的探讨p63与大鼠全脑缺血后神经元凋亡的关系。方法将80只SD大鼠随机分为两组(每组40只):假手术(Sham)组和全脑缺血/再灌注(I/R)组。两组分别在手术后6、12、24、48、72 h处死8只大鼠,行神经行为学评分,检测p63基因表达、神经元密度及凋亡情况。结果 I/R组术后24、48、72 h神经行为学评分较Sham组同时间点增高,差异有统计学意义(P<0.01),其中与同组24、72 h比较,术后48 h最高[(2.6±0.5)分],差异有统计学意义(P<0.05);I/R组海马CA1区p63阳性表达率在术后12、24、48、72 h较Sham组同时间点增高,差异有统计学意义(P<0.01),其中与同组12、24、72 h比较,术后48 h最高[(70.6±9.2)%],差异有统计学意义(P<0.01);细胞凋亡指数较Sham组增加,差异有统计学意义(P<0.01),其中与同组24、72 h比较,术后48 h最高,差异有统计学意义(P<0.05)。结论 p63基因表达在全脑缺血/再灌注后呈先增加后减少的趋势,与神经元凋亡趋势基本一致,提示p63可能参与了神经元的凋亡。

Objective To study the relationship between p63 and neuronal apoptosis after global cerebral ischemia in rats. Methods 80 male SD rats were randomly divided into two groups,40 cases in each group,the sham group and the global cerebral ischemia/reperfusion （I/R） group. Rats were executed for taking cerebral tissues at 6,12,24,48,72 h after operation, 8 cases in each time point. The neurobehavior scores were evaluted and p63 protein expression ,neuronal density and apoptosis in hippocampus CA 1 area were detected. Results The neurobehavior scores at postoperative 24,48,72 h in the I/R group were increased at the same time point compared with the the shamp group （P〈0.01）, which at 48 h was highest, showing statistical difference. The positive expression rate p63 protein of hippocampus CA1 area at postoperative 12,24,48,72 h in the I/R group were significantly higher than that at the same time point in the sham group （P〈0.01）, which at 48h was highest （P〈0.01）, showing statistical difference. The cellular apoptosis index in the I/R group was significantly increased compared with the sham group（P〈0.01 ）, showing statistical difference, maximum was at 48 h compared with that at 24,72 h in the same group, difference showing statistical significance （P〈0.05）. Conclusion The expression of p63 in global cerebral ischemia/reperfusion shows the trend of decreasing firstly and then decreasing, which is basically consistent with neuronal apoptosis trend, suggesting that p63 may be involved in neuronal apoptosis.