摘要
ObjectiveTo 比较描述和羊膜的导出液体的间充质的 stromal 房间(AF MSC ) 和脐的绳索沃顿 .MethodsThe 人 AF MSC 是的导出果冻的间充质的 stromal 房间(WJ MSC ) 的心肌的区别能力从羊水诊断获得的羊膜的液体样品有教养。脐的绳索 WJ MSC 从沃顿由正常劳动完整术语的交付的婴儿的脐的绳索的果冻被获得。由房间表面标记的流动 cytometry 的形态学，生长曲线，和分析在房间的二种类型之间被比较。心肌的基因(GATA-4， c-TnT， α； -actin, 和 Cx43 ) 被即时 PCR 检测，相应蛋白质表情被西方的污点分析检测在以后心肌在 AF MSC 导致了， WJ MSCs.ResultsOur 调查结果表明 AF MSC 和 WJ MSC 分享了 fibroblastoid 形状的类似的词法特征。AF MSC 容易比 WJ MSC 被获得并且有一更短的时间到达 2.7 ± 的坚持；1.6 天到 6.5 ± 的 WJ MSC；1.8 天。由 MTT 细胞毒素的试金的生长曲线证明 AF MSC 在经过 5 和经过 10 点有一个类似的 proliferative 能力。然而， WJ MSC 的 proliferative 能力相对 10 经过在 5 经过被减少。AF 干细胞和 WJ 干细胞与胚胎的干细胞的一些特征有间充质的 stromal 房间的特征。他们表示 CD29 和 CD105，然而并非 CD34。他们为一级主要 histocompatibility 是积极的(MHC 我) 抗原(HLA-ABC ) ，并且否定，或略微积极，为 MHC 班 II (HLA 医生) 抗原。Oct-4 在所有二种房间类型是积极的。AF MSC 和 WJ MSC 能区分心肌层。区别能力被 GATA-4 的表达式检测， c-TnT， α；在心肌的 induction.ConclusionsBoth AF MSC 以后的 -actin, Cx43 和 WJ MSC 在心脏的再生治疗为 myogenesis 有潜在的临床的应用程序。
Objective To compare the characterization and myocardial differentiation capacity of arnniotic fluid-derived mesenchymal stromal cells （AF MSCs） and umbilical cord Wharton＇s Jelly-derived mesenchymal stromal cells （WJ MSCs）. Methods The human AF MSCs were cultured from amniotic fluid samples obtained by amniocentesis. The umbilical cord WJ MSCs were obtained from Wharton＇s Jelly of umbilical cords of infants delivered full-term by normal labor. The morphology, growth curves, and analyses by flow cytometry of cell surface markers were compared between the two types of cells. Myocardial genes （GATA-4, c-TnT, a-actin, and Cx43） were detected by real-time PCR and the corresponding protein expressions were detected by Western blot analysis after myocardial induced in AF MSCs and WJ MSCs. Results Our findings revealed AF MSCs and WJ MSCs shared similar morphological characteristics of the fibroblastoid shape. The AF MSCs were easily obtained than the WJ MSCs and had a shorter time to reach adherence of 2.7 ± 1.6 days to WJ MSCs of 6.5 ± 1.8 days. The growth curves by MTT cytotoxic assay showed the AF MSCs had a similar proliferative capacity at passage 5 and passage 10. However, the proliferative capacities ofWJ MSCs were decreased at 5 passage relative to 10 passage. Both AF stem cells and WJ stem cells had the characteristics of mesenchymal stromal cells with some characteristics of embryonic stem cells. They express CD29 and CD105, but not CD34. They were positive for Class I major histocompatibility （MHC I） antigens （HLA-ABC）, and were negative, or mildly positive, for MHC Class II （HLA-DR） antigen. Oct-4 was positive in all the two cells types. Both AF MSCs and WJ MSCs could differentiate along myocardium. The differentiation capacities were detected by the expression of GATA-4, c-TnT, a-actin, Cx43 after myocardial induction. Conclusions Both AF MSCs and WJ MSCs have the potential clinical application for myogenesis in cardiac regenerative therapy.
