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TNF-α调节鼻咽癌凋亡抑制蛋白表达的体外研究 被引量：1

TNF-α Modulated Inhibitor of Apoptosis Protein Expression of Nasopharyngeal Carcinoma in Vitro
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摘要 [目的]研究TNF-α对鼻咽癌中c-IAP1和c-IAP2表达水平的影响,探索鼻咽癌抗凋亡的机制。[方法]培养鼻咽癌5-8F细胞,经不同浓度(0、1、5、10、20、30ng/ml)的TNF-α处理细胞16h、24h之后,半定量RT-PCR检测c-IAP1和c-IAP2基因表达水平的变化,用WesternBlot检测c-IAP1和c-IAP2蛋白表达水平的变化。[结果]低浓度TNF-α上调鼻咽癌中c-IAP1表达,而高浓度TNF-α下调c-IAP1表达。随着TNF-α浓度增高,c-IAP2表达量不断上调。[结论]TNF-α通过上调鼻咽癌细胞中c-IAP2的表达而使肿瘤细胞抗凋亡,可能成为抗癌靶点。
作者 宋启斌 褚玉新 王桂华
机构地区 武汉大学人民医院肿瘤中心
出处 《中国肿瘤》 CAS 2012年第12期932-934,共3页 China Cancer
关键词 鼻咽癌 TNF-Α 凋亡抑制蛋白
分类号 R739.63 [医药卫生—肿瘤]
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参考文献9

  • 1Wei S,Alajezl NM,Bastianutto C,et al. Significance of Plkl regulation by miR-100 in human nasopharyngeal cancer[J]. Int J Cancer,2010,126(9): 2036-2048.
  • 2Lu X,Qian CN,Mu YG,et al. Serum CCL2 and serum TNF-ct-two new biomarkers predict bone invasion,post- treatment distant metastasis and poor overall survival in nasopharyngeal carcinoma [J]. Eur J Cancer,2011,47(3): 339-346.
  • 3Jin HS,Park HS,Shin JH,et al. A novel inhibitor of apop- tosis protein(IAP)-interacting protein,vestigial-like (Vgl)-4, counteracts apoptosis-inhibitory function of lAPs by nucle- ar sequestration[J]. Biochemical and Biophysical Research Communications. 2011.412(3):454-459.
  • 4夏春波,范才文,黄岚珍,蒋常文.鼻咽癌细胞XIAP mRNA表达及其意义[J].肿瘤基础与临床,2009,22(5):376-377. 被引量：2
  • 5Wu Y,Zhou BP. TNF-oL/NF-KB/Snail pathway in cancer cell migration and invasion [J]. Br J Cancer,2010,102(4): 639-644.
  • 6Taniguchi F,Izawa M,Uegaki T,et al. TNFc+ is a potent mediator of regulating IAP expression in endometriotic cells[J]. Fertility and Sterility,2011,96(3): S138.
  • 7Gyrd-Hansen M, Meier P. IAPs: from caspase inhibitors to modulators of NF-KB,inflammation and cancer [J]. Nature review,2010, 10(8):561-574.
  • 8许杨,赵晓航.IAP家族分子与肿瘤靶向治疗[J].生命科学,2010,22(2):161-168. 被引量：13
  • 9Fulda S. Inhibitor of Apoptosis (IAP) proteins as therapeu- tic targets for radiosensitization of human cancers[J]. Can- cer Treat Rev, 2012,38(6):760-766.

二级参考文献56

  • 1LI Ming SONG Tao YIN Zhen-fei NA Yan-qun.XIAP as a prognostic marker of early recurrence of nonmuscular invasive bladder cancer[J].Chinese Medical Journal,2007(6):469-473. 被引量：19
  • 2Roy N, Mahadevan MS, McLean M, et al. The gene for neuronal apoptosis inhibitory protein is partially deleted in individuals with spinal muscular atrophy. Cell, 1995, 80 (1): 167-78.
  • 3LaCasse EC, Mahoney D J, Cheung HH, et al. IAP-targeted therapies for cancer. Oncogene, 2008, 27 (48): 6252-75.
  • 4Bertrand MJ, Milutinovic S, Dickson KM, et al. cIAP1 and cIAP2 facilitate cancer cell survival by functioning as E3 ligasesthat promote RIP1 ubiquitination. Mol Cell, 2008, 30 (6): 689-700.
  • 5Dai Z, Zhu WG, Morrison CD, et al. A comprehensive search for DNA amplification in lung cancer identifies inhibitors of apoptosis clAP1 and clAP2 as candidate oncogenes. Hum Mol Genet, 2003, 12 (7): 791-801.
  • 6Fong WG, Liston P, Rajcan-Separovic E, et al. Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines. Genomics, 2000, 70 (1): 113-22.
  • 7Konopka K, Spain C, Yen A, et al. Correlation between the levels of survivin and survivin promoter-driven gene expression in cancer and non-cancer cells. Cell Mol Biol Lett, 2009, 14 (1): 70-89.
  • 8Franklin MC, Kadkhodayan S, Ackerly H, et al. Structure and function analysis of peptide antagonists of melanoma inhibitor of apoptosis (ML-IAP). Biochemistry, 2003, 42 (27): 8223-31.
  • 9Eckelman BP, Drag M, Snipas S J, et al. The mechanism of peptide-binding specificity of IAP BIR domains. Cell Death Differ, 2008, 15 (5): 920-8.
  • 10Vaux DL, Silke J. lAPs, RINGs and ubiquitylation. Nat Rev Mol Cell Biol, 2005, 6 (4): 287-97.

共引文献13

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引证文献1

中国肿瘤
2012年 第12期

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