GM-CSF和TNF对人血树突状细胞抗肿瘤活性的影响

Influence of GM-CSF and TNF upon the Anti tumor Activities of Human Dendritic Cells

-观察了新鲜分离和经体外培养 36h的人外周血树突状细胞DC 0和DC 36 ,在GM CSF或TNF作用下 ,辅助淋巴因子和PHA激活的杀伤细胞 (LPAK )体外杀伤人肝癌细胞株BEL 740 2的影响。实验在BEL 740 2、LPAK和DC (DC 0或DC 36 )相混合的基本条件下 ,分别添加GM CSF (5 0 0u/ml,10 0u/ml)或TNF (5 0 0 0u/ml,5 0 0u/ml) ,温育 48h后用中性红比色法检测细胞毒活性。结果显示 ,加入GM CSF各组的细胞毒活性明显增高 (P <0 0 1) ,并在浓度为 10 0u/ml时达到最大效应。加入TNF各组的细胞毒活性也明显增高 (P <0 0 1) ,但存在着剂量依赖关系。DC 36各组与相应的DC 0组比较 ,细胞毒活性明显增高 (P <0 0 1)。表明新鲜分离的DC经 36h培养后 ,其辅助的抗肿瘤活性明显增强 ,与GM CSF和TNF也有更大的协同作用。

The effects of GM CSF and TNF upon the anti tumor activities of freshly isolated dendritic cells in human peripheral blood(DC 0) and those cells cultivated for 36 hours(DC 36) were investigated in vitro Experiments were performed with a mixture of human hepatoma cell line BEL 7402,lymphokine and PHA activated killer cells(LPAK) and dendritic cells(DC 0 or DC 36) in the presence of GM CSF(500u/ml or 100u/ml) or TNF(5000u/ml or 500u/ml) The experimental results showed that the cytotoxic activity was obviously enhanced after addition of GM CSF with the maximal effect observed with 100u/ml;while in case of TNF addition,the cytotoxic activity was also increased,but the effect was concentration dependent In comparison with the DC 0 and DC 36 dendritic cells,the cytotoxic activity of the latter were remakably heightened This effect indicates that the cultivated dendritic cells have a stronger cytotoxic activity than the freshly isolated ones under the cooperative effect with GM CSF and TNF