钾通道基因Kv 1.5在大鼠脑不同发育阶段的mRNA表达研究

Study on the mRNA expression of potassium channel gene Kv1-5 in the developing rat brain

目的 研究大鼠大脑不同发育阶段钾通道基因Ｋｖ１－５的ｍＲＮＡ表达。方法 ①利用一步法提取脑组织中ＲＮＡ；②ＤＮＡ模板的制备；③体外转录合成ＲＮＡ探针；④ＲＮＡ：ＲＮＡ杂交。结果 Ｋｖ１－５在大鼠胎儿期大脑皮层未表达，出生２５ｄ后表达明显增强，成年大鼠与出生后２５ｄ相比表达未发生变化。结论 Ｋｖ１－５ｍＲＮＡ的表达与发育阶段有较大关系，出生前期Ｋｖ１－５ｍＲＮＡ表达快速增长可能与大鼠脑发育和功能分化有关。

AIM To investigate the patterns of potassium channel gene Kv 1 5 mRNA expression in the developing rat brain by using RNase protection assay. METHODS (1) RNA was extracted from brain tissues by one step method, (2) The preparation of DNA template, (3) Synthesis of RNA probe in vitro transcription, (4) RNA:RNA. RESULTS Kv 1 5 was absent in embryo tissue, but significantly increased at postnatal day 25 with adult rat, the mRNA expression did not show any change. CONCLUSION there is close relationship between Kv 1 5 mRNA expression is pertinent to the development and function differentiation of rat brain.