摘要
目的 研究大鼠大脑不同发育阶段钾通道基因Kv1-5的mRNA表达。方法 ①利用一步法提取脑组织中RNA;②DNA模板的制备;③体外转录合成RNA探针;④RNA:RNA杂交。结果 Kv1-5在大鼠胎儿期大脑皮层未表达,出生25d后表达明显增强,成年大鼠与出生后25d相比表达未发生变化。结论 Kv1-5mRNA的表达与发育阶段有较大关系,出生前期Kv1-5mRNA表达快速增长可能与大鼠脑发育和功能分化有关。
AIM To investigate the patterns of potassium channel gene Kv 1 5 mRNA expression in the developing rat brain by using RNase protection assay. METHODS (1) RNA was extracted from brain tissues by one step method, (2) The preparation of DNA template, (3) Synthesis of RNA probe in vitro transcription, (4) RNA:RNA. RESULTS Kv 1 5 was absent in embryo tissue, but significantly increased at postnatal day 25 with adult rat, the mRNA expression did not show any change. CONCLUSION there is close relationship between Kv 1 5 mRNA expression is pertinent to the development and function differentiation of rat brain.
出处
《中国药理学通报》
CAS
CSCD
北大核心
1999年第6期505-508,共4页
Chinese Pharmacological Bulletin
基金
国家杰出青年基金!No 39425014
关键词
钾通道基因
MRNA表达
脑
potassium channel gene Kv 1 5
mRNA expression
rat
brain