维生素E对白细胞功能及免疫功能的影响

Effect of Vitamine E on numbers and functions of leukocytes in peripheral blood and immune function

目的 观察维生素Ｅ（ＶｉｔＥ）对大鼠外周血白细胞数目和功能的影响及对免疫功能的影响。方法 采用ＮＢＴ还原试验法测定白细胞的吞噬功能；采用单向免疫扩散法测定血清ＩｇＧ含量；采用ＣｏｎＡ诱导［３Ｈ］ＴｄＲ参入法测定外周血Ｔ淋巴细胞增殖反应；采用ＥＬＩＳＡ法测定血中γ干扰素（ＩＦＮγ）含量。结果 ＶｉｔＥ（５０－０、１００－０ｍｇ·ｋｇ－１，ｉｇ，３０ｄ）能使经ＣＴＸ诱导的大鼠降低的外周血白细胞计数升高，且能提高白细胞的吞噬能力；还使小鼠经ＣＴＸ诱导降低的ＩｇＧ含量及Ｔ淋巴细胞增殖反应明显升高，血中ＩＦＮγ含量提高。结论 ＶｉｔＥ可使外周血白细胞数量升高，功能增强，并对免疫功能具有调节作用。

AIMS To observe the effect of vitamine E on the numbers and functions of leukocytes in peripheral blood and immune functions. METHODS The method of NBT reduction was adopted to investigate the phagocytic activity of leukocytes; the simple immune diffuse method was applied to measure the concentration of IgG in serum; [ 3H] TdR incorporation method was used to observe the growth of T lymphocytes; the method of ELISA was practiced to measure the concentration of IFN γ in blood. RESULTS VitE (50 mg·kg -1 、100 mg·kg -1 ,ig, 30 d) showed a obvious rise in the numbers of leukocytes which had been decreased by CTX, and promoted the phagocytic activity of leukocytes. In addition, VitE indicated a increase in the concentration of IgG and IFN γ which had been reduced by CTX. Meanwhile, VitE could enhance the growth of T lymphocytes. CONCLUSION The results suggest that VitE enhance either the numbers of leukocytes or the phagocytic activity of leukocytes, and VitE can regulate immune function.