淋巴细胞抑裂素的理化性质及生物活性研究

Studies on the Properties and Bioactivity of Lymphocyte Chalone

本文以猪胸腺为原料 ,经超滤、Biogel p- 4凝胶过滤、DEAE- Sephadex A- 2 5离子交换层析、Sephadex G- 10过滤、HPLC分离等步骤 ,得到分子量小于 30 0 0的多个组分 ,对其中二个组分测定了 N端序列。该抑裂素除可抑制 Con A刺激的淋巴细胞对 3H- Td R的掺入之外 ,体内可抑制机体对异源蛋白的免疫应答反应 ,延迟异体移植皮肤的存活时间 ,还可抑制大鼠同种异体心肺联合移植引起的排斥反应。鉴于抑裂素具有种族非专一性 ,组织专一性 ,非细胞毒和作用可逆性等特点 ,用于治疗自身免疫性疾病 。

A partially purified low molecular weight lymphocyte chalone was obtained from porcine thymus by Biogel P 4,DEAE sephadex A 25 and Sephadex G 10 chromatography. When the partially purified lymphocyte chalone was further separated by HPLC, five peaks were collected.All the five components were active in suppression of mitogen response, but their activities were lower than those partially purified lymphocyte chalone. The partially purified chalone had the capacity to repress the response to heterogenous protein Con A estimated by pad test. Chalone could also markedly prolong the survival period of MHC nonmatched grafted skin in mice from 9 days to 14 days. The results of an experiment of heterotopic heart lung transplantation were exiting. Wistar rats were treated with chalone for two weeks. The transplant operation took place on day 8. Chalone markedly prolonged the survival time of the transplanted heart increased from 8.2 days of the control group to 19.6 days. On day 30, the final day of the experiment, rats in the test group were still alive, but those in the control group already died on 13.2 days. Because of its low molecular weight and species nonspecificity, the lymphocyte chalone isolated from porcine thymus could hopefully be used as an immunosuppressant in the treatment of autoimmune diseases and in prolonging allograft survival, such as in skin,heart, liver, or kidney transplantation.