用Fluo3-AM测定2型糖尿病患者淋巴细胞内Ca^(2+)浓度及药物对患者胞内Ca^(2+)浓度的影响

Determination of the Concentration of Intracellular Calcium in Lymphocyte by Fluo 3 AM and Study of the Influence of the Drugs on Calcium Release

用 Fluo 3- AM为荧光探针 ,测定 2型糖尿病患者淋巴细胞内 Ca2 +浓度 ,观察了肾上腺素、多巴酚丁胺、Bay K 864 4、胰岛素等药物对胞内 Ca2 +的作用机制。实验表明患者胞浆内 Ca2 +浓度与正常人相比显著升高 ;药物对胞内 Ca2 +的刺激表明 ,病人对外界钙激动剂更敏感。胰岛素能刺激胞内 Ca2 +浓度升高 ,其作用机制是激活钙离子通道。肾上腺素不仅能激活钙离子通道 ,也能促使钙从钙池中释放。上述研究也表明 Fluo- 3是有效的研究胞内 Ca2 +变化的荧光探针。 2型糖尿病的发生可能与 Ca2 +浓度升高有关 。

The purpose of the research was to determine the intracellular calcium of patients of NIDDM. We also analyzed the mechanism of several drugs such as Adrenaline, Dobutamine, Bay K 8644 and Insulin which stimulated calcium release of NIDDM, using Fluo 3 AM as fluorescent probe. The results showed that the concentration of intracellular calcium increased compared with healthy persons. Patients are more sensitive to Ca 2+ activator than healthy people. Insulin can stimulate calcium release which mechanism is to excite the Ca 2+ channel. Adrenaline can not only excite the Ca 2+ channel, but also stimulate the calcium release from Ca 2+ pools. The part of Ca 2+ in insulin signal transduction of NIDDM may have defects.