摘要
研究5-氯水杨酰肼三苯基氯化锡(LPY-3)体外抗肿瘤作用及作用机制。采用MTT法检测LPY-3对HO-8910、Hela、A549等6种肿瘤细胞的生长抑制作用;用Western Blotting法检测LPY-3对HO-8910细胞中凋亡相关信号分子及DNA断裂标志蛋白γ-H2AX的诱导作用;用DNA凝胶电泳检测LPY-3对pBR322质粒的DNA断裂作用。结果表明,LPY-3对HO-8910、Hela、A549等细胞有显著的增殖抑制作用,IC50值在0.12～0.26μmol/L浓度范围内;能够诱导HO-8910细胞中凋亡相关信号分子Caspase-9、Caspase-3、Cyt C、PARP剪切,Bcl-2表达降低,γ-H2AX表达升高;对pBR322质粒有DNA断裂作用。
The anti-tumor effects and mechanisms of （Ph2Sn）2（HL9）2（CTHSO2N2）Cl in vitro were in vestigated. MTT assay was used to detect the cytotoxic effect of LPY-3 on six kinds of tumor cell lines. Western Blotting was used to identify the inducements HO-8910 and the symbol protein γ-H2AX about DNA of LPY-3 to the apoptosis signaling molecules in cleavage. Agrose gel electrophoresis was used to detect the cleaving effect of LPY-3 on pBR322. The results indicated that LPY-3 significntly inhibited the growth of these six kinds of tumor cell lines, and their IC50 fall in the 0. 12-0. 26 μmol/L range. LPY-3 can trigger the activation of apoptosis signaling molecules （caspase-3, caspase-9,Cyt C and PARP ）, decrease the expression of Bcl-2 and oppositely advance the expression of γ-H2AX. LPY-3 can cause the DNA cleavage of pBR322.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第12期65-69,共5页
Periodical of Ocean University of China
