去势大鼠骨质疏松症基因表达谱特征初步分析

Preliminary Analysis on Gene Expression Profiles of Osteoporosis in Ovariectomized Rats

目的探讨去势大鼠骨质疏松症与正常大鼠基因表达谱的差异,初步分析去势大鼠骨质疏松症基因表达谱的特征。方法应用基因芯片技术检测去势大鼠骨质疏松症与正常大鼠相关基因的表达,通过Gene Ontology(GO)数据库对差异表达基因进行基因信息学分析,运用Agilent GeneSpring GX 11软件对差异基因进行GO分析。结果去势大鼠骨质疏松症共有118条基因发生了差异表达,其中有48条基因上调,70条基因下调。结论多种基因表达异常与去势大鼠骨质疏松症相关。

Objective To investigate the difference of the gene expression profile between osteoporosis of ovariectomized rats and normal rats, and analyze the characters of the gene expression profile of the osteoporosis of ovariectomized rats. Method The gene expression difference between osteoporosis of ovariectomized rats and normal rats was tested by using cDNA microarray technology. The gene information of the gene expression difference was analyzed in Gene Ontology （GO） database, and the different genes were analyzed by GO analysis using the software Agilent Genespring GXll. Results A total of 118 significant differentially expressed genes were detected, among them, 48 genes were up regulated, 70 were down regulated. Conduslon Many abnormal genes expression related with osteoporosis in ovariectomized rats.