HPLC测定槐花药材不同部位芦丁和槲皮素含量

Content Determination of Rutin and Quercetin in Different Parts of Sophora Japonica L.Medicinal Materials by HPLC

目的比较槐花不同部位(槐花、槐米及枝梗)中芦丁和槲皮素的含量。方法采用高效液相色谱法进行测定,Ultimate XB-C18色谱柱(4.6 mm×250 mm,0.45μm),水-甲醇为流动相梯度洗脱,流速1 mL/min,柱温35℃,检测波长256 nm,进样量20μL。结果芦丁含量为槐米>槐花>枝梗;槲皮素含量整体较低,且槐花>槐米>枝梗。结论槐花各部位芦丁含量较高,且含量差别不大。槐花中的槲皮素含量明显高于槐米及其枝梗。

Objective To compare rutin and quercetin content of different parts （flower, bud and pedicel） of Sophora Japonica L.. Methods HPLC method was used with Ultimate XB-C18 column （4.6 mm×250 mm, 0.45 μm）, water and methanol as solvent system with gradient elution. The flow rate was 1 mL/min, column temperature was 35 ℃, detection wavelength was set at 256 nm, and injection volume was 20 μL. Results The content of rut_in was bud〉flower〉pedicel. The content of quercetin was low on the whole, and in sequence of flower〉bud〉pedicel. Conclusion In the different parts of Sophora Japonica L., rutin content is high with little difference. Quercetin content of flower is significantly higher than in bud and pedicel.