白蜡虫肠道微生物分析及立克次氏体分子检测

Analysis of the Gut Microbes of the Chinese White Wax Scale Ericerus pela and Molecular Detection of Rickettsia

肠道微生物对于昆虫的生长和发育起着重要作用,不仅参与维生素合成、脂肪和碳水化合物的吸收与利用、孤雌生殖、信息素的合成,同时在抵御外来菌的侵入与定植以及在加强免疫系统的功能中也起着重要作用[1-2]。对于刺吸式昆虫来说,由于植物枝干韧皮部汁液营养组成不均衡,碳水化合物含量丰富而必需氨基酸组分欠缺或含量较低[3]。

To understand the diversity of the gut microbes of Chinese white wax scale Ericerus pela, 16S rDNA clonelibrary was constructed to analyze the gut microbes of the female E. pela adults. The results showed that, the gut ofE. pela contained abundant of Rickettsia. Gut microbes Arsenophonus and Variovorax were also been identified. And Rickettsia was the main bacteria in gut of E. pela. The phylogenetic analysis of the 16S rDNA showed that, theRickettsia from E. pela has close relationship with that of pea aphid Acyrthosiphon pisum. PCR amplification was per- formed using the specific primers for Rickettsia to detect infection in 30 male and 30 female individuals. The result showed that, the infected male and female were 24 and 12 respectively.