摘要
肠道微生物对于昆虫的生长和发育起着重要作用,不仅参与维生素合成、脂肪和碳水化合物的吸收与利用、孤雌生殖、信息素的合成,同时在抵御外来菌的侵入与定植以及在加强免疫系统的功能中也起着重要作用[1-2]。对于刺吸式昆虫来说,由于植物枝干韧皮部汁液营养组成不均衡,碳水化合物含量丰富而必需氨基酸组分欠缺或含量较低[3]。
To understand the diversity of the gut microbes of Chinese white wax scale Ericerus pela, 16S rDNA clonelibrary was constructed to analyze the gut microbes of the female E. pela adults. The results showed that, the gut ofE. pela contained abundant of Rickettsia. Gut microbes Arsenophonus and Variovorax were also been identified. And Rickettsia was the main bacteria in gut of E. pela. The phylogenetic analysis of the 16S rDNA showed that, theRickettsia from E. pela has close relationship with that of pea aphid Acyrthosiphon pisum. PCR amplification was per- formed using the specific primers for Rickettsia to detect infection in 30 male and 30 female individuals. The result showed that, the infected male and female were 24 and 12 respectively.
出处
《林业科学研究》
CSCD
北大核心
2012年第6期780-783,共4页
Forest Research
基金
林业公益性行业科研专项经费项目(201204602)
“十二五”国家科技计划课题(2011BAD33B02)
中国林科院中央级公益性科研院所基本科研业务费专项资金项目(riricaf201004M)
国家自然科学基金项目(30771736)资助