格拉司琼与磷钨杂多酸相互作用的共振光散射及分析应用

Resonance Light Scattering of the Interaction of Granisetron with Phosphotungstic Acid and its Analytical Application

在pH 2.2BR缓冲介质中,磷钨杂多酸(PTA)与格拉司琼(GSN)相互作用形成离子缔合物,不仅引起吸收光谱的变化,还导致共振散射光谱(RLS)的显著增强并产生新的RLS光谱,最大RLS峰位于333nm附近,其RLS增强程度与格拉司琼浓度成线性关系,检出限和线性范围分别为12ng/mL和0.04～3.0"g/mL。文中研究了反应产物的吸收和RLS光谱特征,优化反应条件的影响,据此发展了以磷钨杂多酸为光谱探针的灵敏、简便、快速测定格拉司琼的新方法。将方法用于血清中格拉司琼含量的快速定,结果满意。讨论了离子缔合反应和RLS增强机理。

In pH 2. 2 BR buffer solution, Phosphotungstic acid （PTA） and granisetron （GSN） interacted to form ion-association complex, which not only caused the change of absorption spectra, but also led to the resonance light scattering （RLS） markedly in- creased and the new RLS spectrum appeared, and the maximum RLS peak was located in the near of 333 nm. There was a linear relation between the enhancement degree of the scattering intensity and the concentration of granisetron, and the detection limit and the linear range were 12 ng/mL and 0.04-3.0 μg/mL, respectively. The absorption and RLS spectral characteristics of the system were studied; the optimum reaction conditions were discussed. Phosphotungstic acid was chosed as spectral probe, and a new, sensitive, sim- ple and rapid method for determining granisetron was developed. The method had been ap- plied to determination granisetron in human serum samples with satisfactory results. The reaction mechanism and reasons for RLS enhancement were discussed.