两种方法评价常见水果的抗氧化活性

Two Method Evaluating the Antioxidant Activities of Some Common Fruits

分别采用ORAC法(Oxygen Radical Absorbance Capacity)和DPPH·法(2,2-diphenyl-1-picryl-hydrazyl,DPPH)评价十种水果的抗氧化活性,并且分析比较十种水果的抗氧化活性与总酚含量、抗坏血酸含量的相关性。ORAC法测试结果显示:十种水果的抗氧化能力顺序依次为:猕猴桃>橙子>芒果>菠萝>雪莲果>提子>火龙果>香蕉>苹果>梨。十种水果对DPPH.自由基的清除能力由大到小依次为:猕猴桃>雪莲果>橙子>芒果>菠萝>提子>火龙果>香蕉>苹果>梨。尽管两种方法评价的水果抗氧化活性排序有差异,但ORAC法评价结果与DPPH·法评价结果呈较好的相关性(r=0.921,p<0.01)。另外,DPPH.法测定的十种水果抗氧化活性与总酚之间的相关性(r=0.939)强于与抗坏血酸之间的相关性(r=0.8279)。

The antioxidant activities of 10 kinds of fruits were evaluated by Oxygen Radical Absorbance Capac- ity （ORAC） method and DPPH （2,2-diphenyl - pieryl-1-hydrazyl ） method respectively. Meanwhile, the total phenol and ascorbic acid content of these fruits were also determined. A comparative study was conducted in terms of the correlation between their antioxidant activities and their total phenol content and ascorhic acid content as well. Results from ORAC method indicated the sequence of antioxidant capacity of the 10 kinds is： kiwi 〉 orange 〉 mango 〉 pineapple 〉 yaeon 〉 grapes 〉 pitaya 〉 banana 〉 apple 〉 pear. The capacity of free rad- ical scavenging of the 10 fruits determined by DPPH was ranged as ： kiwi 〉 yacon 〉 orange 〉 mango 〉 pineap- ple 〉 grapes 〉 pitaya 〉 banana 〉 apple 〉 pear. Results indicated that the results from ORAC method and DPPH method were well correlated （r =0. 921, p 〈0.01 ） in spite of some minor differences in terms of the rates of the antioxidant activities of different fruits. And results from DPPH method indicated the correlation （ r = 0. 939 ） between total phenol content and the antioxidant activities was stronger than the correlation （ r = 0.8279） between ascorbic acid content and the antioxidant activities.