高白细胞性急性白血病临床表现与免疫表型研究

Analysis of the clinical manifestations and the immune phenotypes of high white blood cell acute leukemia

目的分析、比较各型高白细胞性急性白血病患者临床症状和白细胞分化的免疫表型,为该疾病的诊断、预后等提供依据。方法采用流式细胞术,对高白细胞性急性淋巴细胞性白血病(HLALL)和高白细胞性急性骨髓系白血病(HLAML)(共25例)、非高白细胞性急性淋巴细胞性白血病(NHLALL)和非高白细胞性急性髓系白血病(NHLAML)(共85例)的临床表现及免疫表型做比较分析。结果与NHLA比较,HLAL发病呈现急性及髓外发展的临床特征;对白血病细胞表面免疫学标记研究发现CD13和CD33为髓系细胞较特异表型,而HLALL可以同时出现多种免疫表型。结论 HLAL发病呈现急性、髓外发展且预测性差,CD33和CD13在白细胞免疫分型的髓系细胞中为较特异标志。

This article was designed to analyze the clinical manifestations and the immune phenotypes of high white blood cell acute leukemia. Flow cytometry assays were used for the comparative analysis of clinical manifestations of HLALL and HLAML （25 cases）, NHLALL and NHLAML （85 cases） and phenotype. Results showed that, compared with the NHLA, HLAL incidence presented clinical features of acute and extramedullary development with CD33 and CD13 positive myeloid antigen expression; however, there were variable markers simultaneously expressed in HLALL cases. Results from this study indicate that HLAL disease present with acute and extramedullary development and poor predictability, while HLALL cell heterogeneity is much higher than other groups and CD33 and CD13 expressions were relatively stable in myeloid myeloid cells.