糖皮质激素性青光眼患者小梁细胞体外培养和超微结构研究

Culture and ultrastructure of trabecular cells from patients with glucocorticoid induced glaucoma

目的 探索糖皮质激素性青光眼患者小梁细胞体外培养方法及超微结构的特点。方法从小梁切除术中取得的巩膜内板层 ,应用组织学培养方法进行患者小梁细胞体外培养及鉴定 ,并将糖皮质激素性青光眼患者小梁细胞与正常小梁细胞的超微结构进行分析和比较。结果 糖皮质激素性青光眼患者小梁组织培养的细胞经鉴定确为小梁细胞 ,但与正常小梁细胞相比 ,其细胞的微绒毛、吞饮小泡及胞浆的溶酶体含量较少。结论 糖皮质激素性青光眼患者小梁细胞体外培养成功使小梁细胞培养体系更加完善 。

Objective To culture the trabecular cells from patients with glucocorticoid induced glaucoma (GIG) and study their ultrastructures Methods The trabecular specimens from trabeculectomy were cultured in vitro and passaged for 3 times, then identified Moreover, the glaucomatous cells were compared with the normal ones under electron microscope Results Cultured trabecular cells from patients with GIG were obtained The ultrastructure of these cells showed the decrease in microvilli, pinosomes and cytoplasmic lysosomes Conclusions The establishment of human glaucomatous trabecular cell culture in vitro makes the culture system more perfect The morphologic changes might be related to the abnormal functions of human trabecular meshwork cells