支原体通用PCR引物的设计及在诊断的应用

DESIGN OF UNIVERSAL PRIMERS FOR THE PCR DETECTION OF MYCOPLASMA AND THEIR CLINICAL APPLICATION

目的 快速全面检测感染人体、动物和细胞培养的多种常见支原体。方法 从 12种支原体的 16S与 2 3SrRNA核酸序列中选择了两段高度保守的核酸序列 ,作为支原体通用引物 ,一次PCR扩增就能检测出 12种支原体。结果 用这对通用引物检测了 41份临床标本 ,16份细胞培养物和 2 0只大白鼠 ,阳性率分别为 14 6 %、 43 7%和 2 0 0 %。结论 用通用引物检测支原体具有覆盖面广、检测种数多、不易发生漏检等优点。

In order to detect Mycoplasma species mainly infected in human,animal and cell cultures,Universal PCR primers were designed according to the published sequences of 16s and 23s rRNA of Mycoplasma、More than ten culture of different Mycoplasma species were detected by PCR with the universal primers Resulted in the designed profiles in electrophoresis,41 Vagiuna-Swab samples,30 wistars and 16 cell cultures were also 14 6%、20 0% and 43 7% respectively,It showed the positive PCR detection of Mycoplasma with universal primers could be a helpful way in laboratory and clinical