狂犬病毒抗原刺激小鼠的细胞免疫反应试验

CELLULAR IMMUNE RESPONSE IN MICE INDUCED BY RABIES VIRUS ANTIGEN

为了研究狂犬病毒抗原刺激机体所产生的细胞介导免疫反应的抗病毒作用 ,我们利用小鼠进行试验。注射环磷酰胺 (Cy)的小鼠再接种狂犬疫苗和稀释液 ,然后将其脾细胞转移到 2 4小时前脑内接种 1或 10 0 0LD50 狂犬病毒标准攻击毒(CVS)的同系小鼠 ,结果表明小鼠的脾细胞不仅可以转递抗狂犬病毒攻击的能力 ,而且可以转移“早期死亡”现象 ;另一方面 ,注射Cy的小鼠接种狂犬病毒抗原 ,两周后用CVS攻击 ,结果发现接种狂犬病毒抗原的小鼠有一定程度的保护作用 ,但中和试验结果阐明这些小鼠体内没有产生中和抗体 ,从而说明狂犬病毒抗原刺激所产生的细胞免疫作用有一定抗狂犬病毒攻击的能力。

Different features of cellular immune response induced by rabies virus antigen were studied in mice Mice were given with cyclophosphamid (cy) Two days later mice received (intra peritoneal) inactivated rabies vaccine or equivalent placebo All mice were killed 2,3,4 or 5 days later Their splenocytes were harvasted and transferred (intravenous) into one syngenic recipient which had been challenged,24 hours before,with 1 LD 50 or 1000 LD 50 (intracerebral) of rabies virus The result showed that ability to resist later challenge can be transferred,to syngeneic mice,simply via splenocytes of cy-treated mice,but “early death” phenomena can be transferred (from one syngenic mouse to another) via splenocytes of vaccinated animals transferred into mice inoculated with large amounts of rabies virus On the other hand,mice were injected with rabies virus antigen after they had been given cy Two weeks later these mice were challenged by CVS We found that these mice can produce a certain extent protective action But the result of neatralizing test indicated that mice were not induced neatralizing antibodies in vivo These studies preliminarily proved that cellular immune response induced with rabies virus antigen can activate special ability to resist later rabies virus challenge