白羽半番肉鸭坦布苏病毒的分离鉴定及结构蛋白基因分析

Identification and Sequence Analysis of Tembusu Virus Isolated from White-feather Mule Ducks

自肝脏表现不规则出血的白羽半番肉鸭脏器中分离获得1株病毒(命名为ZZ40株),经(RT-)PCR检测为鸭坦布苏病毒,可被鸭坦布苏病毒高免阳性血清特异性中和。应用鸭坦布苏病毒特异性引物从该株病毒基因组中成功获得结构蛋白基因片段,该片段与近年来我国分离的禽源坦布苏病毒株核酸序列同源性均在99.1%以上,而与蚊媒源坦布苏病毒MM-1775株同源性仅为88.6%;分子进化分析表明该株病毒与BYD-1等近年来的鸭坦布苏病毒分离株共处于一个进化分支。以上结果表明,我国白羽半番肉鸭存在坦布苏病毒感染,且该分离毒株的抗原性与我国早期分离于种(蛋)鸭的坦布苏病毒差异不明显。

A viral isolate（named as ZZ40） was abtained from liver of white-feather mule ducks with irregular hemorrhagic hepatitis,and were identified as duck tembus virus（DTMUV） by RT-PCR.The virus was specifically neutralized by anti-DTMUV hyperimmune serum.Gene fregments were abtained by using three sets of primers aimed at viral structural genes,and shared no less than 99.1% nucleotide sequence identity with those of avian tembus viruses isolated recently,and no more than 88.6% with that of Mosquito-borne tembus virus MM-1775 strain.Phylogenetic analysis showed that ZZ40 strain clustal into the same clade with those duck viruses isolated recently.These results indicated that the white-feather mule ducks in this case was infected with duck tembusu virus,which has no abviously variation in antigenicity compared with those of the early isolates of duck tembusu virus from egg-laying duck.