茶树杂交F_1代儿茶素类的遗传表型研究

Genetic Phenotype of Catechins in Tea Plants Hybrid F_1 Generation

以高EGCG茶树新品系为主要杂交亲本,分析其杂交F1代儿茶素类的遗传表型。结果表明:杂交F1代的EGC、EC、EGCG、ECG、酯型儿茶素类、简单儿茶素类、TC(儿茶素总量)、酯型儿茶素类/TC、简单儿茶素类/TC、EGCG/咖啡碱、EGCG/TC、儿茶素(类)品质指数等指标,不同株系差异很大;不同杂交组合存在显著差异,Kruskal Wallis检验和稳健检验均达显著性水平;不同杂交组合的分离存在极显著性差异,方差齐性检验表明达到极显著性水平。不同杂交组合F1代TC的分布因亲本的遗传力而定;不同杂交组合F1代高EGCG单株、高酯型儿茶素类单株和高儿茶素(类)品质指数单株的分布也因亲本的遗传力而定。

The hybrid F1 generation of the new tea plants with high EGCG content were used in this experimentation to study the genetic phenotype of catechins.The results showed that（a） the contents of EGC,EC,EGCG,ECG,ester-catechins,simple catechins and TC（total catechins）,the ratios of ester-catechins/TC,simple catechins/TC,EGCG/caffeine and EGCG/TC,as well as the catechin quality index of the tea plants differed significantly among different varieties;（b） the differences among the hybrid combinations reached a significant level according to Kruskal Wallis and the robust tests;and（c） the different hybrid combinations separated very significantly from one another based on the homogeneity of variance test.The TC distribution in the F1 generation of the hybrid combinations was determined by the genetic heritability.The distribution of individual plants with high EGCG,high ester-catechins and high quality catechins index was also determined by the heritability of their parents′ genetic.