米诺环素经腹主动脉灌注对兔脊髓缺血再灌注损伤的保护作用

目的建立兔脊髓缺血再灌注损伤模型，研究经腹主动脉灌注米诺环素对脊髓缺血再灌注损伤的作用。方法新西兰大耳白兔16只，随机分为A、B两组，各8只，A组为对照组，B组为米诺环素组。静脉麻醉后气管插管，持续监测平均动脉压、心率、脉搏血氧饱和度及直肠温度。手术暴露腹主动脉和双侧髂总动脉，经股动脉置一细导管至腹主动脉内距左肾动脉1．0cm处，于左肾动脉开口远端0．5cm阻断腹主动脉，同时阻断左、右髂总动脉，阻断即刻开始经导管向阻断的腹主动脉远端以12ml·kg^-1·h^-1的速度灌注实验药物0．5h，A组灌注常温生理盐水，B组为等容量米诺环素溶液（10mg／kg），30min后开放血管。于动物完全清醒即刻、再灌注后6、24、48h对双后肢神经功能进行评估；再灌注48h，取脊髓L3-5 3个节段制作石蜡切片，光镜观察并计数正常前角运动神经元。结果清醒即刻、再灌注后6、24、48h，B组神经行为学评分明显高于A组（P〈0．05）；再灌注48h，A、B两组脊髓前角正常神经元中位数（P25-P75）分别为1（0～2）、8（4～11）。B组明显高于A组（P〈0．05）。结论腹主动脉阻断期间动脉内灌注米诺环素可减轻脊髓缺血再灌注损伤。

Objective To make a rabbit model of ischemia-reperfusion injury in spinal cord model and explore the treatment for the ischemia-reperfusion injury in spinal cord by arterial perfusion of minocycline through abdominal aorta. Methods 16 New Zealand white rabbit were randomly divided into group A and B with 8 in each group. A group was control group and B group with minocycline treatment. A catheter was put through femoral artery to the abdominal aorta and abdominal aorta together with left and right common iliac artery were occluded, physiological saline and minocycline （ 10mg/kg ） was perfused to the distal side of abdominal aorta respectively in group A and B, the occlusion was released 30min later. The nerve function was evaluated at the time the rabbit returned to consciousness, 6h,24h, 48h after reperfusion; three segments of spine core L3-5 were taken out and made into paraffin sections for observation and counting of normal angulus anterior motoneuron under microscope. Results The score of neuroethology of B group was significantly higher than that of A group at the time of returning to consciousness, 6h,24h, 48h after reperfusion （ P〈0.05 ） ; the median of counting of normal angulus anterior motoneuron （ P25-P75 was ） 1 （ 0-2 ） , 8 （ 4-11 ） , which was much higher in B group thanAgroup （ P〈0.05 ） .