大鼠蛛网膜下腔出血后NR2B在脑组织中的表达

目的观察大鼠蛛网膜下腔出血（subarachnoidhemorrhage，SAH）后N-甲基-D-天门冬氨酸受体亚单位NR2B在脑组织中的表达变化。方法42只成年健康雄性SD大鼠，随机分为正常组、对照组及SAH后1d（D1）、3d（D3）、5d（D5）、7d（D7）、14d（D14）共7组，各6只。SAH模型制作：经大鼠自体尾动脉（股动脉）非肝素化动脉血注入视交叉池，分别在蛛网膜下腔出血后1、3、5、7、14d处死，取大脑皮层、海马、小脑做标本，Western blot及免疫组化测定NR2B。结果对照组与正常组相比差异无统计学意义；SAH后1d组NR2B蛋白水平明显降低（P〈0．01），SAH后3、5、7d组NR2B蛋白水平降低（P〈0．05），SAH后14d组NR2B蛋白水平与正常组相比差异无统计学意义。结论NR2B在SAH后24h明显下降，之后逐渐上升，至14d时基本恢复正常。

Objective To observe the expression changes of NR2B subunit of the N-methyl-D-aspartate receptor in the brain tissue after subarachnoid hemorrhage （ SAH ） . Methods 42 male Sprague Dawley rats were randomly divided into seven group, i.e.normalgroup, control group, 1 days （D1） , 3 days （D3） , 5 days （D5） , 7days （D7） , 14 days （ D 14 ） after SAH. All SAH animals were subjected to injection of 0.3ml fresh arterial non-heparinized blood into prechiasmatic cistern. Brain samples （ cerebral cortex, hippocampus, cerebellum ） were extracted in 1, 3, 5, 7, 14days after SAH. The expressions of NR2B were measured by western blot and immunohistochemistry. Results NR2B proteins were low-regulated significantly following SAH （ P〈0.01 ） .There was no statistically significant difference between normal group and control group （ P〉0.05 ） .The decrease activity of NR2B in 1 day after SAH was markedly reduced in animals of the normal group （ P〈0.01 ） . Compared with the normal group, D3, D5, D7, D14 group had reduced （ P〈0.05 ） , D14 group had no significant difference （ P〉0.05 ） . Conclusion NR2B at 24 hours after SAH decreases significantly, then gradually rises, and returns to normal in 14 days.