紫薯色素与防腐剂复配抑菌作用

Bacteriostasic activity of purple sweet potato pigment compound with preservatives

对紫薯色素进行提取、纯化,做抑菌试验,对金黄色葡萄球菌的最小抑菌浓度(MIC)为1.25mg.mL-1。在单因素实验的基础上,将紫薯色素作为抑菌物质与山梨酸钾、苯甲酸钠进行复配做抑菌实验,用响应面法对三者复配比进行优化。结果表明,紫薯色素与苯甲酸钠交互作用对金黄色葡萄球菌抑菌作用极显著。复配物最佳抑菌配比为:山梨酸钾0.480mg.mL-1,苯甲酸钠0.260mg.mL-1,紫薯色素2.15mg.mL-1。在此条件下,对金黄色葡萄球菌的实际抑菌圈直径为24.7mm。

The anthocyanins from purple sweet potato was extracted and purified.The minimum inhibitory concentration（MIC） on S.aureus was selected to be 1.25 mg·mL-1.We compounded purple sweet potato pigment with potassium sorbate and soudium benzoate to do bacteriostatic experiments,whose proportin was optimized by using the response surface methodology.Our results showed that the interaction between purple sweet potato pigment and soudium benzoate was significant factor to antimicrobial activity,and the optimum compound proportion was： potassium sorbate 0.480 mg·mL-1,soudium benzoate 0.260 mg·mL-1 and purple sweet potato pigment 2.15 mg·mL-1.Under these conditions,the inhibition zone diameters of E.coli was up to 24.7mm.