不同蛋白酶水解模拟餐厨垃圾优化工艺研究

Study on Optimal Hydrolyzation Condition of the Simulated Food Waste with Different Proteases

[目的]研究不同蛋白酶水解模拟餐厨垃圾的优化工艺。[方法]通过单因素试验,用牛肉模拟餐厨垃圾,研究了木瓜蛋白酶、中性蛋白酶、碱性蛋白酶、胰蛋白酶和胃蛋白酶水解牛肉的优化工艺。[结果]较其他几种酶,胰蛋白酶水解效果最好,水解度最大可达到74.18%。胰蛋白酶水解的最适条件:酶用量2%,pH 7.5,固液比为1∶60,水解时间2 h,水解温度50℃。[结论]该研究为解决餐厨垃圾的同源性污染问题打下了基础。

[Objective] The research aimed to study optimal hydrolyzation condition of the simulated food waste with different proteases.[Method] Via single-factor test,beef as the simulated food waste,the optimal technics of papain,neutrase,alcalase,trypsin and pepsin hydrolyzing beef were studied.[Result] Compared with other proteases,the maximum hydrolysis degree for trypsin was 74.18%,and its hydrolysis effect was the best.The optimal hydrolysis condition of trypsin was 2% of enzyme dosage,pH 7.5,1∶60 of solid-liquid ratio,2 h of hydrolysis time and 50 ℃ of hydrolysis temperature.[Conclusion] The research laid foundation for solving homology pollution problem of the food waste.