反相高效液相色谱法测定吉非罗齐片中主药的含量

Determination of Gemfibrozil Tablets Content by HPLC

建立反相高效液相色谱(RP-HPLC)法测定吉非罗齐片中主药的含量。色谱柱为安捷伦的C18柱,以甲醇-缓冲盐(体积比为75∶25)为流动相,检测波长276nm,流速1.0mL/min,测定吉非罗齐片中主药的含量。结果显示吉非罗齐和2,5-二甲酚达到完全分离。在考察吉非罗齐的质量浓度范围内具有良好的线性关系,r达到0.999,平均回收率为99.81%,RSD为0.89%。可见用所建立的分析方法测定吉非罗齐片中主药的含量,结果准确、可靠。

A method was established for the determination of Gemfibrozil tablets by HPLC. The Agilent C18 column was used. The detection wavelength was 276 nm. The mobile phase was methanol-water （mass ratio of 75：25）, and the flow rate was 1.0 mL/min. The Gemfibrozil and 2,5-xylenol had good resolution, and the linear ranges of Gemfibrozil were good （r=0. 999）. The average recoveries were 99. 81%. The proposed method is simple and accurate, and it can be used for the determination of Gemfibrozil tablets.