铁皮石斛组培苗生根条件优化研究

Rooting condition optimization of Dendrobium officinale plantlets

【目的】培养生根优良的铁皮石斛组培苗,提高移栽成活率。【方法】在生根培养基中添加不同激素配比(1.0～3.0mg/LNAA、0.5mg/L6-BA)进行铁皮石斛组培苗生根培养,并采取正交试验考察光照强度、pH和琼脂用量对铁皮石斛组培苗生根效果的影响。【结果】随着MS培养基中NAA浓度的升高,试管苗的鲜重和生根率呈逐渐降低的趋势,以添加NAA1.0mg/L(处理1)和NAA2.0mg/L(处理3)的试管苗长势最好。极差分析和方差分析结果表明,在3个培养条件中,对试管苗生根的影响大小表现为光照强度>琼脂用量>pH值,生根培养基3因素最优水平组合为光照强度2000lx,pH5.5,琼脂用量8.0g/L。【结论】铁皮石斛试管苗适宜的生根培养基为1/2MS+10%香蕉泥+NAA1.0mg/L+2%蔗糖+0.05%活性炭,适宜的生根条件为光照强度2000lx、琼脂用量8.0g/L、pH5.5。

[Objective ]This research cultured excellent Dendrobium officinale plantlets in order to improve the survival rates of transplanting plantlets. [ Method ] In the rooting culture medium of Dendrobium officinale with different hormone combinations （1.0-3.0 mg/L NAA, 0.5 mg/L 6-BA）, the orthogonal experiments of light intensity, pH, and agar amounts were adopted to test the Dendrobium o[Eeinale rooting effects. [Result]As the NAA concentration in MS medium increased, the fresh weight of test-tube plantlet and its rooting rate gradually decreased, and adding NAA 1.0 mg/L （treatment 1 ） and NAA 2.0 mg/L （treatment 3） were optimal for test-tube plantlet growth. From the range and variance analysis results, under the 3 culturing conditions, the effects on Dendrobium offlcinale were ranked in the order of illumination〉agar amount〉pH values, and the optimal combination of the 3 factors for rooting medium was A3B2C3. [Conclusion]The optimal Dendrobium officinale rooting plantlets medium was 1/2MS＋10% mashed banana＋NAA 1.0 mg/L＋2% cane sugar＋0.05% active carbon, and the suitable rooting conditions were light intensity 2000 lx, 8 g/L agar content, and pH level 5.5.