应用正交试验设计优选番木瓜组培苗生根培养基研究

Rooting medium optimization of Carica papaya plantlets by using orthogonal design

【目的】研究培养基及其不同成分对番木瓜组培苗生根诱导的效果,为番木瓜组培快繁提供依据。【方法】以台农一号番木瓜侧芽组培第8代继代苗为材料,接种到含不同基本培养基配比、不同IBA、NAA、活性炭(AC)浓度的生根培养基上进行生根培养,采用L(934)正交试验设计优选生根培养基。【结果】在MS、1/2MS和3/2MS培养基中诱导的番木瓜组培苗生根率存在显著差异,诱导效果表现为MS>1/2MS>3/2MS;不同IBA浓度对番木瓜生根率影响效果表现以2.0mg/LIBA>2.5mg/LIBA>IBA1.0mg/LIBA,以2.0～2.5mg/L为宜;不同NAA浓度对番木瓜生根的诱导作用表现为0.05mg/L>0.10mg/L>0mg/L,以0.05mg/L为宜;活性炭对番木瓜生根诱导作用表现为0.002%AC>0.005%AC>0.000%AC,浓度以0.002%为宜;MS培养基配比、IBA、NAA、AC对番木瓜生根率影响大小表现为IBA>AC>NAA>MS培养基配比。【结论】最佳诱导番木瓜生根培养基配方为MS+2.0mg/LIBA+0.05mg/LNAA+0.002%AC。

Objective This research studied the different compositional effects of rooting medium on rooting induction of Carica papaya plantlets in order to provide references for further tissue culture and rapid propagation.Method Using the eighth generation tissue culture plantlets＇ lateral buds of the Carica papaya Tainong No.1 as test materials in this experiment,the plantlets were inoculated to the correspondent matching culture medium;different concentrations of IBA,NAA,and AC were applied as the rooting medium for rooting culture,and the best rooting medium were selected by using the orthogonal experiment design L9（34）.Result In the MS,1/2MS,and 3/2MS culture medium,the rooting rate of plantlets showed significant difference,and the induction effects were ranked in the following order：MS1/2MS3/2MS.Different rooting rates of papaya with various IBA concentrations were ranked in the following order：2.0 mg/L2.5 mg/L 1.0 mg/L IBA and the optimal IBA concentration ranged between 2.0-2.5 mg/L.Different NAA concentrations on the rooting induction of papaya were ranked in the following order：0.05 mg/L 0.10 mg/L 0.00 mg/L,and the optimal concentration was 0.05 mg/L.The activated carbon effects on the rooting induction of papaya were ranked in the following order：0.002%0.005%0.000%,and the optimal concentration was 0.002%.Lastly,the MS culture medium,IBA,NAA,and AC on the papaya rooting rate were ranked in the following order：IBAACNAAMS culture medium.Conclusion The optimal medium combination for papaya rooting induction was MS＋2.0 mg/L IBA＋0.05 mg/L NAA＋0.002% AC.