人参强力胶囊中六种人参皂苷HPLC测定方法的建立及评价

Establishment and Evaluation of the HPLC Method for Determination of Six Ginsenosides in Renshen Qiangli Capsules

建立测定人参强力胶囊中6种人参皂苷(Rg1、Re、Rb1、Rc、Rb2、Rd)含量的HPLC方法。色谱柱为Alltima C18(250mm×4.6mm,5μm)柱,流动相为乙腈-0.02%磷酸水溶液,流速为1.0mL/min;梯度洗脱;检测波长为203nm;人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rd线性范围分别为0.396 8～7.936μg/mL、0.406 4～8.128μg/mL、0.196 0～3.920μg/mL、0.204 0～4.080μg/mL、0.200 0～4.000μg/mL、0.201 6～4.032μg/mL,相关系数均大于0.999 0。6种人参皂苷的平均加样回收率在100.1%～103.4%之间,RSD均小于1.5%。本方法简便、快捷、准确,可以用于人参强力胶囊的质量控制。

To develop an HPLC method for determination of six active ginsenosides（Rgl ,Re,Rbl ,Rc,Rbz,Rd）in Renshen Qiangli Capsules. Chromatographic separation was performed on an Alhima C18 （250mm × 4.6mm,5μm）column; mobile phase was acetonitrile- 0.02 % phospho- ric acid, flow rate was 1.0mL/min; gradient elution; detector wavelength was 203nm. Good linear relationships of six ginsenosides were provided over investigated concentration ranges. The linear relationships of six ginsenosides Rgl, Re, Rbl, Rc, Rbz, Rd were 0.396 8 - 7.936μg/mL, 0.406 4 - 8.128txg/mL, 0.196 0 - 3.920μg/mL, 0.204 0 - 4.080μg/mL, 0.200 0 - 4.000μg/mL, 0.201 6 - 4.032μg/mL, respectively. The values of correlation coefficient were higher than 0.999 0 for all the analytes. The average recoveries of the six components ranged from 100.1% to 103.4 %. Repeatability experiments showed that relative standard deviation（ RSD ）values of the six contents were less than 1.5 %. The developed method was simple, sensitive and repeatable, and can be used for quality control of Renshen Qiangli Capsules.