毛蕊异黄酮葡萄糖苷大鼠在体肠吸收动力学的研究

Study on the Absorption Kinetics of Glucosidase Calycosin in Rat Intestines

目的研究毛蕊异黄酮葡萄糖苷肠吸收动力学特征。方法以酚红为标示物,采用大鼠在体单向肠灌流实验,以超高效液相色谱法(UPLC)测定灌流液中药物的含量,研究毛蕊异黄酮葡萄糖苷在大鼠不同肠段吸收特性,并考察不同药物浓度对大鼠肠吸收的影响。结果毛蕊异黄酮葡萄糖苷在十二指肠段的吸收明显高于其他肠段,增加药物浓度,毛蕊异黄酮葡萄糖苷在十二指肠的吸收速率常数基本保持不变。结论毛蕊异黄酮葡萄糖苷在整个肠段都有不同程度的吸收,且在十二指肠的吸收速率最快,其吸收机制为被动扩散。

OBJECTIVE To investigate the absorption kinetics of glucosidase calycosin in intestines.METHODS The rat single-pass intestinal perfusion experiments were taken with the marker phenol red,and the content of the drug in the perfusate was determined by the method of UPLC（Ultra Performance Liquid Chromatography）.The study then focused on the absorption characteristics of glucosidase calycosin in rat＇s different intestinal segments and the effect of different drug concentrations on absorption.RESULTS The effect of glucosidase calycosin on the absorption of duodenum segment was apparently higher than other segments.The calycosin glucosidase absorption rate constant was essentially unchanged in the duodenum,even if the concentration of drug was increased.CONCLUSION The glucosidase calycosin can be absorbed in the whole intestine segments with different absorptivity,and in the duodenum segment is the highest with the mechanism of passive diffusion.