晚发性抑郁症患者认知功能及其与载脂蛋白E基因多态性的关联研究

Association study between cognitive function and apolipoprotein E gene polymorphism in patients with late-onset depression

目的:探讨晚发性抑郁症(LOD)患者认知功能及其与载脂蛋白E(APOE)基因多态性的关联。方法:LOD患者97例,健康对照组44例,采用聚合酶链式反应-限制性片段长度多态性检测APOE基因多态性。所有受试者完成了汉密尔顿抑郁量表评估及神经心理学测试。结果:患者组的神经认知测试成绩除连线测试外,其余测试成绩都显著低于正常对照组(P<0.001);APOE基因型和等位基因频率分布两组间差异无统计学意义。在控制年龄、教育和性别后,以APOE基因是否携带ε4等位基因将受试者分为ε4+组和ε4-组,分析APOEε4等位基因对受试者认知功能的影响,以及ε4等位基因和抑郁症的诊断交互作用对认知功能的影响。结果发现,APOEε4等位基因对LOD患者认知功能无明显的主效应,APOEε4等位基因和抑郁症的诊断之间无交互作用影响认知功能。结论:LOD患者存在广泛的认知功能受损,APOE基因多态性与LOD的发病及认知功能未发现有显著关联。

Objective：To study the relationship between apolipoprotein E（APOE） gene polymorphism and late-onset depression（LOD） patients and cognition function.Method：The polymorphism of APOE gene was analyzed by polymerase chain reaction and restriction fragment length polymorphism genotyping assay in 97 LOD patients and 44 healthy controls.The Hamilton depression scale and neuropsychological tests were used to assess depressive severity and cognitive function of all subjects,respectively.Results：The performances of neuropsychological tests in LOD group except TMT were significantly poorer than those in control group（P0.001）.There were no significant differences between LOD and controls on genotype and allele frequencies of APOE gene.The subjects were divided into ε4＋ group and ε4-group by whether or not apolipoprotein E gene carrying ε4 allele.After controlling factors such as age,education level and gender,the impacts on cognitive function for subjects,both apolipoprotein Eε4 allele and the interaction between ε4 allele and depression diagnosis,were analyzed.The results showed that apolipoprotein Eε4 allele made no main effect on cognitive function for patients with late onset depression.And the interaction between ε4 allele and depression diagnosis also didn＇t show any effect on cognitive function for subjects.Conclusion：The LOD patients in acute phase might have extensive impairments of cognitive function,and APOE gene polymorphism was not found significant correlation.