口腔变形链球菌感受态刺激因子的蛋白合成及活性检测

Chemical synthesis and biological activities of competence stimulated peptide of quorum sensing system in Streptococcus mutans UA159

目的探讨口腔变形链球菌(S.mutans)密度感应系统信号蛋白的体外合成及其生物活性。方法采用化学合成法口腔S.mutans密度感应感受态刺激因子(CSP)信号蛋白,通过高效液相色谱仪纯化合成蛋白,质谱分析结构,通过扫描电镜对比观察CSP信号蛋白对S.mutans生物膜形成的影响,分析CSP信号蛋白的生物活性。结果化学合成纯化S.mutans CSP蛋白分子,加入CSP信号肽后,S.mutans生物膜呈团状密集分布,细菌之间存在厚实的黏性胞外分泌物,细菌黏连呈链团状。结论成功合成口腔S.mutans CSP信号蛋白,该蛋白肽具备促进S.mutans生物膜形成的生物活性。

Objective To synthesize and analyze the biological activities of the competence stimulated peptide （CSP） in Streptococcus mutans （S.mutans） quorum sensing system. Methods The CSP peptide derived from S.mutans UA159 comC gene N-terminal 26-46 was chemically synthesized and purified. The biological activity of CSP, which was used as a quorum-sensing signal in S.mutans biofihn formation CSP, was analyzed by scanning electron microscope （SEM）. Results The CSP was accurately synthesized, and SEM confirmed the addition of chemically synthetic CSP induced the adhesion of S.mutans cells and significantly increased the biofilm formation of S.mutans. Conclusion The CSP was chemically synthesized with biological activities, which made it possible to study the mechanism of quorum sensing system in S.mutans.