还阳参醇提物止咳祛痰平喘及抗炎作用的实验研究

Antitussive,expectorant,anti-asthma,and anti-inflammatory effects of alcohol extracts from Crepis turczaniowii

目的探讨还阳参醇提物对实验动物的止咳、祛痰、平喘及抗炎作用。方法分别采用氨水引咳法、气管酚红排泄法、组织胺致喘息法观察还阳参醇提物的止咳、祛痰、平喘作用;采用二甲苯致小鼠耳肿胀和蛋清致大鼠足趾肿胀法观察还阳参醇提物抗急性炎症的作用;气管内注入脂多糖法建立大鼠慢性支气管炎模型,观察受试物对模型大鼠肺组织的病理学改变。结果还阳参醇提物能显著减少小鼠的咳嗽次数、增加小鼠气道酚红排泄量、延长组织胺所致豚鼠喘息的潜伏期、可减轻蛋清所致的大鼠足趾肿胀度,与对照组比较差异具有显著性(P<0.05或0.01);还阳参醇提物能减轻慢性支气管炎大鼠黏膜上皮细胞的坏死和脱落,抑制黏膜层和黏膜下层炎性细胞浸润。结论还阳参醇提物有明确的止咳、祛痰、平喘及抗炎作用,对慢性支气管炎大鼠模型的支气管黏膜有保护作用。

Objective To identify the effects of the alcohol extracts from Crepis turczaniowii（AECT）,such as antitussive,expectorant,anti-asthma,and anti-inflammation,on the experimental animals.Methods The cough models of mice were established by ammonia liquor,Phenol Red excretion,and histamine;The acute inflammation models of mice were made by xylene and albumen;The models of chronic bronchitis of rats were made by intratracheal instillation of lipopolysaccharide to observe the lung pathematology.Results AECT could reduce the cough frequency of mice,increase the excretion of phenol red,prolong the latency of asthma induced by histamine,reduce the paw edema extent of rats induce by albumen,compared with the control groups（P0.05,0.01）.The histopathological examination revealed AECT could reduce the mortification and desquamation of mucosal epithelial cells in rats with chronic bronchitis and inhibited the mucous and submucosa infiltration of inflammatory cells.Conclusion AECT have the antitussive,expectorant,anti-asthma,and anti-inflammatory effects and could protect the bronchial mucosa of rats with chronic bronchitis.