基于指纹图谱技术探索不同粒径连翘粉体中化学成分溶出规律

Study on dissolution characteristic of chemical constituents in Forsythiae Fructus powders with different particle diameters based on fingerprinting techniques

目的建立不同粒径连翘粉体的高效液相色谱法(HPLC)特征指纹图谱,并对其进行系统聚类分析,探讨粒径与化学成分溶出特征的相关性。方法将连翘药材进行粉碎、过筛,按粒径大小分成10组,采用HPLC法建立不同粒径连翘粉体的指纹图谱,结合主要共有指纹峰信息,对不同粒径连翘粉体进行系统聚类分析。结果不同粒径连翘粉体共有8个HPLC特征峰,根据各粉体单位质量峰面积,不同粒径连翘粉体被分为不同层次的3类,其中超微粉单位质量峰面积最大。结论不同粒径连翘粉体与化学成分溶出具有良好的相关性,超微粉碎技术可以促进有效成分的溶出,在粉碎加工药材方面具有很好的应用前景。

Objective To establish the chromatographic fingerprint of Forsythiae Fructus powder （FFP） with different particle diameters and to explore the correlation between the particle diameter and the dissolution characteristic of chemical constituents by hierarchical cluster analysis. Methods Forsythiae Fructus was crushed, filtered, and divided into ten groups according to particle diameter. The chromatographic fingerprint of FFP with different particle diameters was established by HPLC. FFP with different particle diameters was analyzed by hierarchical cluster analysis. Results The eight characteristic peaks were identified in FFP with different particle diameters. And they could be divided into three categories according to peak area per unit mass. The biggest peak area per unit mass was supper micropowder. Conclusion There are good correlations between particle diameter and dissolution characteristic of chemical constituents. Superfine pulverizing technology could be used to promote the dissolution rate of chemical constituents and have a good practical future in the crushing process of crude drugs.