T10对LPS诱导脑内炎症反应中血脑屏障的保护作用

The Protective Effects of Triptolide on Blood Brain Barrier in Cerebral Inflammation Induced by LPS

目的:研究雷公藤甲素对内毒素诱导的脑内炎症中血脑屏障通透性,神经细胞的保护作用。方法:选用健康成年雄性SD大鼠,随机分为雷公藤甲素处理组(T10+LPS组),内毒素组(LPS组),生理盐水组(NS组),每组6只动物。常规Nissl染色、GFAP免疫组化染色和伊文思蓝(EB)荧光示踪法。结果:尼氏染色显示NS组海马CA1区锥体神经元排列规则整齐。LPS组的神经元细胞的密度和层次较NS组和T10+LPS组少,且锥体神经元数目减少,排列散乱,细胞间距加大,神经元有明显的丢失;GFAP免疫组化染色结果显示,NS组海马CA1区GFAP免疫阳性细胞分布稀疏,胞体较小,突起细长,染色较浅。LPS组较T10+LPS组星形胶质细胞密集,胞体和突起大,染色深;伊文思蓝荧光示踪结果,LPS组的大脑皮质与海马结构以及脑血管周围的EB荧光强度都明显强于T10+LPS组和NS组;T10+LPS组与NS组EB荧光强度无差别。结论:T10在LPS诱导的神经炎症中对神经元有保护作用,其机制可能与保护BBB有关。

Objective： To investigate the effects of triptolide on the permeability of blood brain barrier and protection of neurocytes during cerebral inflammation induced by LPS. Methods： Healthy adult male SD rats were divided into 3 groups： triptolide group （T10＋LPS）, LPS group, NS group, with 6 rats in each group. Regular Nissl＇s staing,GFAP immunohistochemical staing and Evans blue fluorescence tracer method were used in the present study. Results： The results of Nissl＇s staining showed that in NS group, the pyramidal cells in the hippocampal CA1 region arranged regularly and the loss of neurons was unremarkable. In LPS group, either the density or the layers of both neurons and pyramidal cells was less than that in （T10＋LPS） group or NS group. Neurocytes of the rats in LPS group scattered randomly and the intercellular spaces increased. For immunohistochemical staining of GFAP, it revealed that the staining positive cells distributed sparsely in the hippoeampal CA1 region of the rats in NS group, which had smaller cell bodies and slender processes, and also were stained more lightly. Both the number and color depth of the positive cells increased more obviously in LPS group or （T10＋LPS） group compared to that in NS group. The number of astrocytes increased, the cell bodies and processes were bigger, and the color of the stained cells was darker in LPS group than those in （T10＋LPS） group. In the analysis of Evans blue fluorescence staining, the intensity of EB fluorescence in cerebral cortex and hippocampus formation, and around the cerebral vessels was higher than that in （T10 ＋LPS） group or NS group. However, there was no significant difference between the results of EB fluorescence staining of（T10＋LPS） group and NS group. Conclusion： The study suggests that T10 may influence the function of BBB and reduced the inflammation reaction of astrocytes to protect the neurocytes from impairment during cerebral inflammation process induced by LPS.