黑龙江省稻瘟病菌无毒基因分析及抗病种质资源筛选

Analyzing Avirulence Genes of Magnaporthe oryzae from Heilongjiang Province and Screening Rice Germplasm with Resistance to Blast Fungus

采用7个稻瘟病菌无毒基因的特异性引物,对177个来自黑龙江省的稻瘟病菌单孢菌株进行PCR检测,结果表明7个无毒基因在黑龙江省不同地区均有分布,但出现频率不同,其中频率最高的为ACE1,达到61.6%,最低的为Avr1-co39,只有31.6%;来源于不同水稻品种的菌株无毒基因组成不同。采用国际水稻研究所20个已知抗性基因的单基因系对48个黑龙江省稻瘟病菌进行毒力分析,与PCR检测无毒基因结果一致。同时,对20个抗病单基因系采用多菌株人工接种发现Pi-9在黑龙江6个地区对稻瘟病菌的抗谱为80%~100%,在育种中具有较高利用价值。

The avirulence genes from 177 single spore strains of Magnaporthe grisea isolated from different rice varieties in Heilongjiang Province were detected by specific primers. The results indicated that Avr-pita, ACE1, Avr-pia, Avr-pit, Avr1-co39, Avr-pik, and Avr-pizt could be detected in the strains from different rice-growing regions, and the amplification frequencies of avirurence genes were associated with the geographical orgin and rice variety, ACE1 and Avr1-co39 showed the highest and lowest frequency, that was 61.6% and 31.6%, respectively. Moreover, 20 monogenic lines with known blast resistance genes from International Rice Research Institute （IRRI） were used to analyze the genes which were valuable in breeding program in Heilongjiang Province by artificial inoculation. The resistance gene Pi-9 had the broadest resistance spectrum to the isolates from six regions.