过量表达鲁氏酵母耐盐基因GPD1对酿酒酵母的影响

Effect of Overexpression of Salt-tolerant Gene GPD1 on Saccharomyces cerevisiae

GPD1是3–磷酸甘油脱氢酶的编码基因,在酵母的耐盐通路中发挥着重要的作用.为了探讨耐盐酵母家族成员鲁氏酵母的GPD1对酿酒酵母的影响,本文利用分子生物学方法构建了质粒YEplac195-GPD1,并将其转入到酿酒酵母菌株W303中,得到工程菌株WYS.研究发现在菌株WYS中过量表达鲁氏酵母的GPD1,其耐盐性、抗性及甘油产量均明显高于对照菌株WY,特别是在含盐量为18%的情况下菌株WYS甘油产量提高了14.35%.说明GPD1的过量表达是菌株耐盐性提高的重要原因.

GPD1 encoded glycerol-3-phosphate dehydrogenase plays an important role in the salt-tolerant pathway of yeast. Zygosaccharomyces rouxi belongs to the salt-tolerant family. In order to analyze the effect of ZrGPD1 （GPD1 gene of Zygo- saccharomyces rouxi）on the salt-tolerance of Saccharomyces cerevisiae,plasmid YEplac195-GPD1 was constructed and then transformed into Saccharomyces cerevisiae W303, forming the engineered strain WYS. The results showed the the salt tolerance, resistance and the production of glycerol in the strain WYS were improved by overexpression of GPD1 compared with that of the control strain WY. Noticeably, the production of glycerol in the strain WYS was raised by 14.35% under the condition of 18% salt. The data suggested that overexpression of GPD1 was the important reason for the improvement of salt tolerance.