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两个X-STR基因座变异亲子鉴定1例

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摘要 1案例资料1.1简要案情及DNA分型检验因申报户口,需要鉴定某男与1名女孩是否存在亲生血缘关系。采集某男、女孩以及其生母的末梢血液。用Chelex-100法提取DNA,采用Identifiler试剂盒(AB公司)进行15个常染色体STR基因座检测,并使用Investigator Argus X-12试剂盒(QIAGEN公司)进行12个X-SrrR基因座检测。实验操作按照使用说明书进行,在ABI9700型基因扩增仪进行复合扩增,产物在ABI3130基因分析仪上进行毛细管电泳,分别采用LIZ500及BTO550分子量标准为内标,用GeneMapper ID v3.2软件进行基因分型。
出处 《中国法医学杂志》 CSCD 2012年第6期502-503,共2页 Chinese Journal of Forensic Medicine
基金 福建省卫生厅青年科研课题(2011-1-31)
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