应用MALDI-TOF-MS法筛选儿童急性淋巴细胞白血病潜在标记物

Identification of New Markers for Childhood Acute Lymphoblastic Leukemia by MALDI-TOF-MS

本研究旨在分析儿童急性淋巴细胞白血病(c-ALL)的肿瘤特异性蛋白,寻找新的肿瘤蛋白分子标志物。应用双向凝胶电泳(2-DE)及基质辅助激光解吸电离飞行时间质谱分析(MALDI-TOF-MS)及联网检索数据库,鉴定表达上调的差异蛋白,并应用细胞免疫组织化学法验证相关蛋白。结果表明,获得了4个表达上调的蛋白点,经质谱分析初步鉴定出其中2个表达上调的差异蛋白为谷胱甘肽S转移酶P和阻殖蛋白;细胞免疫组织化学法检测发现,二者在儿童ALL细胞内的表达阳性率明显高于对照组,差异具有统计学意义(P<0.05)。结论:谷胱甘肽S转移酶P和阻殖蛋白有望成为儿童ALL新的诊断标志物和药物靶点。

The aim of this study was to identify new biomarkers for early diagnosis of childhood acute lymphoblastic leukemia （ALL）. The total protein samples of ALL cells and normal peripheral lymphocytic cells were extracted respectively. The two-dimensional gel electrophoresis （2-DE）, matrix-assisted laser desorption ionization time- of- flight-mass spectrometer （MALDI-TOF-MS） and database searching were used to identified differentially upregulated proteins, the S-P immunohistochemical method was performed for confLrming and validating these proteins. The results indicated that two differentially upregulated proteins were preliminarily identified as glutathione S-transferase P（GST-P） and prohihitin （PHB） in ALL group. The immunohistochemistry detection revealed that the positive ratio of GST-P/ PHB in ALL group was statistically higher than that in control group. It is concluded that GST-P and PHB may become the promising diagnostic biomarkers and drug targets for childhood oatients with ALL.