膜联蛋白A2基因对多发性骨髓瘤细胞的诱导凋亡作用及相关机制研究

Apoptosis-inducing Effect of Annexin A2 on Multiple Myeloma Cells and Its Related Mechanisms

本研究探讨膜联蛋白A2(AnxA2)基因诱导骨髓瘤U266、RPMI8226细胞凋亡的作用及相关机制。采用AnxA2 siRNA转染人骨髓瘤U266、RPMI8226细胞,应用实时PCR和Western blot检测AnxA2基因和蛋白的表达。应用流式细胞术检测细胞凋亡,应用实时PCR检测凋亡相关基因的表达水平。结果表明,AnxA2siRNA转染U266和RPMI8226后,AnxA2基因和蛋白表达水平均明显下调;细胞凋亡率增高(P<0.05),同时降低凋亡相关基因p65NF-κB、IL-2、IL-6的表达(P<0.05),增强P53基因的表达(P<0.05)。结论:AnxA2基因沉默在骨髓瘤细胞U266和RPMI8226凋亡中起促进作用。

This study was purposed to investigate the apoptosis-inducing effect of Annexin A2 gene （AnxA2） on multiple myeloma （MM） cells and its mechanisms. The human MM cell lines U266 and RPMI8226 were transfected by using siRNA targeting at AnxA2; the expressions of AnxA2 mRNA and protein in the siRNA-transfected cells were detected by real-time PCR and Western blot, respectively; the cell apoptosis was assayed by flow cytometry. The results showed that silencing AnxA2 gene by siRNA resulted in decreased expressions of AnxA2 gene and protein, increased apoptosis of U266 and RPMI8226 cell lines （ P 〈 0.05 ）, at the same time resulted in down-regulation of apoptosis- related gene expressions including p65NF-κB, IL-2, IL-6 （ P 〈 0.05 ）, and up-regulation of P53 gene expression （ P 〈 0. 05 ）. It is concluded that the AnxA2 silence plays a promoting role in apoptosis of MM cell lines U266 and RPMI8226.