MCF-7荷瘤小鼠血清及树突状细胞上清液中炎性细胞因子水平变化及意义

Significance of detecting cytokines levels in serum and dendritic cells-derived supernatans in MCF-7 mice

目的探讨MCF-7荷瘤小鼠模型血清和树突状细胞(dendritic cells,DCs)上清液中细胞因子水平的变化,为免疫治疗提供依据。方法裸鼠(对照组)及MCF-7裸鼠模型(荷瘤组)各10只,应用ELISA法检测血清及DCs上清液中肿瘤坏死因子(tumor necrosis factor,TNF)-α、白细胞介素(interleukin,IL)-6、IL-12水平。结果荷瘤组血清TNF-α水平((314.81±103.36)pg/mL)低于对照组((371.87±115.30)pg/mL),但2组比较差异无统计学意义(P>0.05),DCs上清液TNF-α水平((1.88±0.10)ng/mL)高于对照组((1.72±0.07)ng/mL)(P<0.05);荷瘤组血清IL-6水平((170.72±54.51)pg/mL)高于对照组((122.35±20.65)pg/mL)(P<0.05),DCs上清液IL-6水平((57.18±5.06)pg/mL)高于对照组((44.53±4.86)pg/mL)(P<0.05);荷瘤组血清及DCs上清液IL-12水平((119.80±33.53)pg/mL和(89.76±8.89)pg/mL)均低于对照组((186.22±51.91)pg/mL和(157.98±48.31)pg/mL)(P<0.05)。结论 MCF-7荷瘤小鼠血清IL-6可能参与该类乳腺癌肿瘤细胞的生长;DCs可能参与产生TNF-α,促进IL-6分泌,抑制IL-12分泌,对肿瘤细胞的杀伤作用减弱。

Objective To detect cytokines levels in serum and dendritic cells （DCs）-derived supernatans in MCF 7 mice in order to provide a basis for immunotherapy. Methods The levels of tumor necrosis faetor-a （TNF a）, interleukin （IL）-6 and IL-12 were detected serum and DCs derived supernatans with ELISA in 10 MCF-7 mice （tumor-bearing mice） and 10 nude mice. Results The level of TNF-a in serum was （314. 81± 103. 36） pg/mL in tumor bearing mice and （371.87±115.30） pg/mL in nude mice, which showed no significant difference （P〉0.05）. And the level of TNF-a in DCs-derived supernatans was （1.88 ± 0. 10）ng/mL in tumor-bearing mice and （1. 72 ± 0. 07） ng/mL in control mice, which showed a significant difference （P^0.05）. The IL-6 levels in serum and DCs-derived supernatans were （170.72± 54. 51） pg/mL and （57. 18 ± 5. 06） pg/mL in tumor-bearing mice, significantly higher than those in nude mice （（122.35±20. 65） pg/mL and （44. 53±4. 86） pg/mL） （P〈0.05）. The IL-12 levels in serum and DCs-derived supernatans were （119.80± 33.53） pg/mL and （89.76 ± 8.89） pg/mL in tumor bearing mice, significantly lower than those in nude mice （（186.22-1-51.91） pg/mL and （157.98-48.31） pg/mL） （P〈0.05）. Conclusion IL 6 in serum may participate breast cancer cell growth in MCF-7 tumor-hearing mice. DCs may participate producing TNF-a, promoting the secretion of IL-6 and inhibiting the secretion of IL 12, and may decrease the ability of killing tumor cell.