Bmi-1和EZH2基因在皮肤血管瘤组织中的表达及意义

Expression and signifieance of Bmi-1 and EZH2 in Human Dermal Hemangiomas

目的探讨Bmi-1和EZH2基因在血管瘤组织中的表达及其意义。方法收集武汉大学人民医院病理科2008年-2011年皮肤毛细血管瘤存档蜡块40例,其中男性15例,女性25例。采用免疫组织化学S-P法检测40例皮肤血管瘤增生期、退化期及正常皮肤组织Bmi-1和EZH2基因表达水平,采用HPIAS-1000图文报告管理系统对Bm i-1和EZH2基因的表达进行定量分析,并用SPSS11.5软件对各组免疫组织化学反应阳性颗粒的平均光密度、阳性面积率做单因素方差分析和SNK(q)检验。结果 (1)Bmi-1的表达增生期血管瘤血管内皮细胞中可见密集分布的棕黄色颗粒,Bmi-1呈高表达,正常皮肤组及退化组血管内皮细胞中可见少量的棕黄色颗粒,Bmi-1呈低表达。增生期组Bmi-1的表达明显高于退化期组和正常皮肤组(P<0.05),而后两组比较差异无统计学意义(P>0.05)。(2)EZH2的表达增生期血管瘤血管内皮细胞中可见密集分布的棕黄色颗粒,EZH2呈高表达,正常皮肤组及退化组血管内皮细胞中可见少量的棕黄色颗粒,EZH2呈低表达。增生期组EZH2的表达明显高于退化期组和正常皮肤组(P<0.05),而后两组比较差异无统计学意义(P>0.05)。结论 Bm i-1和EZH2基因在血管瘤增生期均呈高表达,表明Bmi-1和EZH2基因均参与了血管瘤的发生和发展。

Objective To investigate the effect of Bmi-1 and EZH2 in human dermalhemangioma. Methods Specimens from 40 cases （15 males and 25 females） of hemangioma were collected from the De- partment of Pathology in Renmin Hospital of Wuhan University from 2008 to 2011. We examined the ex- pression of Bmi-1 and EZH2 in proliferative, involuting hemangioma and normal skin tissues by using im- munohistochemical technique. Average optical density and positive area rates of expression of Bmi-1 and EZH2 were measured by image analysis （HPIAS-1000）. Results 1. Bmi-1 expression was significantly higher in the proliferative phase of angioma in endothelial cells than in involuting hemangioma and normal skin （P^0.05）. No significant difference was found between the expression of Bmi-1 in involuting heman- gioma and that in normal skin tissues （P^0. 05）. 2. EZH2 expression was significantly higher in the pro- liferative phase of angioma in endothelial cells than in involuting hemangioma and normal skin （P〈0.05）. No significant difference was found between the expression of EZH2 in involuting hemangioma and that in normal skin tissues （P〉0.05）. Conclusion Bmi-1 and EZH2 expressions high in proliferating hemangio- ma, indicating that both Bmi-1 and EZH2 are engaged in the occurrence and development of hemangioma.