不同浓度亳菊水提物对秀丽隐杆线虫脂滴凝聚影响观察

Effects of Aqueous Extract from Flos Chrysanthemi of Boju on lipidosis of Caenorhabditis elegans

目的探讨亳菊对秀丽隐杆线虫脂肪沉积的影响。方法用不同浓度亳菊水提取液配制NGM培养基,培养同步化秀丽隐杆线虫,成虫经油红O染色,异丙醇萃取,测定OD值,并与普通NGM培养基培养的同步化线虫相比较。结果随着浓度的提高,线虫体内的脂肪沉积量呈现出一定的曲线变化,其具体机制有待于进一步研究。

Objective To explore the influence of the aqueous extract from Flos Chrysanthemi （the flower of Chrysanthemum morifolium Ramat. ） Boju on lipidosis of Caenorhabditis elegans. Methods Dif- ferent concentrations of the aqueous extract from Boju were used to prepared NGM media for nematodes. After oil red O staining and isopropyl alcohol extraction, OD values were detected by spectrophotometer. Results The absorbency value showed a certain curve which changed with the increase in the concentration, and its mechanism needs to be studied further.