期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

亚砷酸钠对胰岛β细胞的Nrf2及其调控的抗氧化酶表达的影响 被引量：3

Effects of sodium arsenite on expressions of Nrf2 and antioxidant in pancreatic β-cells
下载PDF
导出
摘要 目的检测亚砷酸钠对小鼠胰岛β细胞的核转录因子Nrf2及其调控的抗氧化酶硫氧还蛋白(Srx),γ-谷氨酰半胱氨酸连接酶催化亚基(Gclc)表达的影响。方法应用2、4、6、8、10μmol/L亚砷酸钠(NaAsO2)作用MIN6细胞6h,通过Western Blot检测Nrf2蛋白表达;利用Real-time PCR检测抗氧化酶Srx、Gclc的mRNA表达。结果 4μmol/L亚砷酸钠染毒6h后,Nrf2的蛋白表达显著增高,并随着亚砷酸钠剂量的增大,Nrf2蛋白表达也随之增多。抗氧化酶Srx、Gclc mRNA在2μmol/L亚砷酸钠染毒6h后开始出现明显的诱导表达,并随亚砷酸钠剂量的增加而表达增强。结论亚砷酸钠能诱导小鼠胰岛β细胞Nrf2蛋白及其调控的抗氧化酶Srx和Gclc基因的表达,并存在显著地剂量依赖性。 Objective To investigate the expressions of Nrf2 and its regulated downstream antioxidases induced by sodium arsenite （NaAsO2） in mouse pancreatic H-cell MIN6. Methods MIN6 cells were exposed to control（medium） or 2, 4, 6, 8, 10/~mol/L NaAsO2 for 6h. The protein expression of Nrf2 was detected by Western Blot. The mRNA expressions of antioxidases were measured by Real-time PCR. Results The protein level of Nrf2 was significantly increased by 4, 6, 8, 10#mol/L NaAsO2 for 6h. The induction of some antioxidant genes, including sulfiredoxin （Srx） and 7-glutamate cysteine ligase catalytic subunit （Gclc）, was markedly enhanced in a dose-dependent fashion. Conclusion The expressions of Nrf2 and its regulated downstream antioxidases Srx and Gclc can be induced dose-dependently by sodium arsenite in mousue pancreatic β-cell MIN6.
作者 杨蓓 丁金兰 陈雪
机构地区 中国医科大学基础医学院组织与胚胎学教研室
出处 《中国组织化学与细胞化学杂志》 CAS CSCD 2012年第6期574-577,共4页 Chinese Journal of Histochemistry and Cytochemistry
关键词 亚砷酸钠 核转录因子Nrf2 抗氧化酶 MIN6 Sodium Arsenite Nrf2 Antioxidant MIN6
分类号 R329 [医药卫生—人体解剖和组织胚胎学]
  • 相关文献

参考文献9

  • 1Steinmaus C, Yuan Y, Liaw J, et al. Low-level Population Exposure to Inorganic Arsenic in the United States and Diabetes Mellitus: A Reanalysis. Epidemiology, 2009, 20(6) : 807-815.
  • 2Longnecker MP. On Confounded Fishy Results Regarding Arsenic and Diabetes. Epidemiology, 2009,20 (6) : 821-823.
  • 3Diaz-Villaseor A, Burns AL, Salazar AM, et al. Arsenite reduces insulin secretion in rat pancreatic beta-cells by decreasing the calcium-dependent calpain-10 proteolysis of SNAP-25. Toxicol Appl Pharmacol, 2008,231 (3) : 291-299.
  • 4Xue P, Hou Y, Zhang Q, et al. Prolonged inorganic arsenite exposure suppresses insulin-stimulated AKT 8473 phosphorylation and glucose uptake in 3T3-L1 adipocytes: involvement of the adaptive antioxidant response. Biochem Biophys Res Commun,2011,407(2) :360-365.
  • 5Shi H, Shi X, Liu KJ. Oxidative mechanism of arsenic toxicity and carcinogenesis. Molecular and Cellular Biochemistry. 2004, 255(1-2):67-78.
  • 6Itoh K,Wakabayashi N, Katoh Y, et al. Keapl regulates both cytoplasmic-nuclear shuttling and degradation of Nrf2 in response to electrophiles. Genes Cells, 2003,8 (4): 379-391.
  • 7Taguchi K, Motohashi H, Yamamoto M. Molecular mechanisms of the Keapl-Nrf2 pathway in stress response and cancer evolution. Genes Cells, 2011, 16(2) 1123-140.
  • 8Jingbo Pi, Wei Qu, Jeffrey M Reece, et al. Transcription factor Nrf2 activation by inorganic arsenic in cultured kerati- nocytes: involvement of hydrogen peroxide. Exp Cell Res, 2003, 290(2) :234-245.
  • 9李冰,刘洁,李昕,张新玉,朱博,刘丹,邢晓越,孙贵范.亚砷酸钠对核转录因子Nrf2和血红素单加氧酶-1表达的影响[J].环境与健康杂志,2009,26(12):1058-1060. 被引量：11

二级参考文献10

  • 1Sun G. Arsenic contamination and arsenicosis in China [J]. Toxicol Appl Pharmacol, 2004, 198:268-271.
  • 2Flora SJ. Arsenic-induced oxidative stress and its reversibility following combined administration of N-acetylcysteine and meso 2,3-dimereaptosuccinic acid in rats [J]. Clin Exp Pharmacol Physiol, 1999, 26:865- 869.
  • 3Wild AC, Moinova HR, Mulcahy RT. Regulation of gammaglutamylcysteine synthetase subunit gene expression by the transcription factor Nrf2 [J ]. J Biol Chem, 1999, 274:33627-33636.
  • 4Kobayashi M, Yamamoto M. Molecular mechanisms activating the Nd2- Keapl pathway of antioxidant gene regulation [J]. Antioxid Redox Signal, 2005, 7: 385-394.
  • 5Venugopal R, Jaiswal AK. Nrfl and Nrf2 positively and c-Fos and Fral negatively regulate the human antioxidant response elementmediated expression of NAD (P)H:quinone oxidoreductasel gene [J]. Proc Natl Acad Sci U S A, 1996, 93: 14960-14965.
  • 6Barchowsky A, Klei LR, Dudek E J, et al. Stimulation of reactive oxygen, but not reactive nitrogen species, in vascular endothelial cells exposed to low levels of arsenite [J]. Free Radic Biol Med, 1999, 27:1405- 1412.
  • 7Pi J, Yamauchi H, Kumagai Y, et al. Evidence for induction of oxidative stress caused by chronic exposure of Chinese residents to arsenic contained in drinking water [J]. Environ Health Perspect, 2002, 110: 331-336.
  • 8Nguyen T, Sherratt PJ, Pickett CB. Regulatory mechanisms controlling gene expression mediated by the antioxidant response element [J]. Annu Rev Pharmacol Toxicol, 2003, 43:233-260.
  • 9Pi J, Qu W, Reece JM, et al. Transcription factor Nrf2 activation by inorganic arsenic in cultured keratinocytes: involvement of hydrogen peroxide [J]. Exp Cell Res, 2003, 290: 234-245.
  • 10Aono J, Yanagawa T, Itoh K, et al. Activation of Nrf2 and accumulation of ubiquitinated A170 by arsenic in osteoblasts [J]. Biochem Biophys Res Commun, 2003, 305: 271-277.

共引文献10

同被引文献10

  • 1Wang SL, Chang FH, Liou SH, et al. Inorganic arsenic exposure and its relation to metabolic syndrome in an in-dustrial area of Taiwan. Environ Int, 2007, 33(6):805- 811.
  • 2Rahman M, Wingren G, Axelson O. Diabetes mellitus among Swedish art glassworkers-an effect of arsenic ex- posure. Scan& J. Work Environ. Health, 1996, 22 (2) :146-149.
  • 3Nabi AH, Rahman MM, Islam LN. Evaluation of Bio- chemical Changes in Chronic Arsenic Poisoning among Bangladeshi Patients. Int J Environ Res Public Health, 2005, 2(3-4) :385-393.
  • 4Navas-Aeien A, Silbergeld EK, Pastor-Barriuso R, et al. Arsenic exposure and prevalence of type 2 diabetes in US a- dults. JAMA, 2008, 300(7):814-822.
  • 5Druwe IL, Vaillaneourt RR. Influence of arsenate and arsenite on signal transduction pathways: an update. Arch Toxicol, 2010, 84(8) ：585-596.
  • 6Itoh K,Wakabayashi N, Katoh Y, et al. Keapl regu- lates both cytoplasmic-nuclear shuttling and degradation f Nrf2 in response to eleetrophiles. Genes Cells, 2003, (4):379-391.
  • 7Taguchi K, Motohashi H, Yamamoto M. Molecular mech- anisms of the Keapl-Nr{2 pathway in stress response and cancer evolution. Genes Cells, 2011, 16(2):123-140.
  • 8杨蓓,陈雪,丁金兰.Nrf2在急性亚砷酸钠暴露的小鼠胰岛β细胞中的表达[J].解剖学报,2013,44(3):345-348. 被引量：2
  • 9杨蓓,陈雪,丁金兰.RNAi介导的Nrf2表达下调对亚砷酸钠诱发的小鼠胰岛β细胞毒性损伤的影响[J].中国组织化学与细胞化学杂志,2013,22(5):401-405. 被引量：1
  • 10吕嘉诚,金壮壮,杨蓓.tBHQ对亚砷酸钠致胰岛β细胞氧化应激损伤的影响[J].解剖科学进展,2018,24(3):310-313. 被引量：5

引证文献3

二级引证文献6

中国组织化学与细胞化学杂志
2012年 第6期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部