WWOX蛋白在胰腺癌组织中的表达及其临床意义

Expression and clinical significance of WWOX protein in human pancreatic cancinoma tissues by tissue microarray

目的探讨WWOX蛋白在胰腺癌组织中的表达及其临床意义。方法应用免疫组织化学S-P法检测人胰腺癌组织芯片150芯(包括70例胰腺癌组织和5例胰腺炎)中WWOX蛋白的表达,采用HPIAS-1000图文报告管理系统对WWOX蛋白的表达进行定量分析,并用SPSS11.5软件对各组免疫组织化学反应阳性颗粒的平均光密度、阳性面积率做单因素方差分析和SNK(q)检验。结果胰腺癌组织中WWOX蛋白呈低表达,胰腺炎组织中WWOX蛋白呈高表达。结论提示WWOX基因表达下调可能在胰腺癌的发生、发展中起了重要作用。

Objective To detect the expression and clinical significance of WWOX proteins in human Pancreatic cancinoma tissues and pancreatitis tissues. Methods We examined the expression of WWOX pro- teins in human Pancreatic cancinoma and pancreatitis tissues with microarray of 150 chips （70 cases of pan- creatic carcinoma and 5 of pancreatitis） by using immunohistochemical technique, The results of immuno- histochemical dyeing were analyzed by multi-spectral imaging systems to perform quantitative analysis of WWOX protein. Analysis of variance （ANOVA） and SNK（q） test were used with SPSS13.0 to analyze the data from quantum dot dyeing（a=0.05）. Results The expression of WWOX proteins was low in hu- man pancreatic cancinoma tissues, but high expression in pancreatitis tissues. The difference was statisti- cally significant （P〈 0.05）. Conclusion The down-regulatio of WWOX gene expression may play an im- portant role the occurrence and development of pancreatic cancinoma .